欧美日韩在线免费,色黄视频,调教一区

LASCPC-01

規格:

價格:

編號:B161409

品牌:Mingzhoubio

標準菌株

定量菌液

DNA

RNA

規格:

凍干粉

斜面

甘油

平板

加入購物車

在線咨詢收藏

產品名稱

LASCPC-01

商品貨號

B161409

Organism

Homo sapiens, human

Tissue

prostate

培養基

Product Format

frozen 1.0 mL

Morphology

aggregate

Culture Properties

suspension/aggregate

Biosafety Level

2 [Cells contain lentivirus genes]

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease

prostate cancer

Age

75-80

Gender

male

Ethnicity

Caucasian

Applications

This cell line with the N-Myc as a driver of NEPC can serve for studying therapeutic treatments of Neuroendocrine prostate cancer (NEPC)

Karyotype

Conventional karyotyping: normal 46 X,Y
Array CGH: mosaic gian of 1q23.1-1q44

Images

Cell Micrograph of LASCPC-01 cells, ATCC CRL-3356

Clinical Data

75-80 years
male
Caucasian

Comments

Expression of RFP, GFP, MYCN, and myristoylated AKT1.

Complete Growth Medium

To prepare 500mL of complete medium:

464 mL Modified HITES medium (prepared as below)
1 mL Hydrocortisone (Sigma cat# H-0135; 10 nM or 1.0 x 10^-8 M)
5 mL Transferrin (Sigma cat# T-5391; 0.01 mg/mL or 10 µg/mL)
25 mL FBS (ATCC 30-2020)
5 mL L-Glutamine (ATCC 30-2214)

Modified HITES Medium

Insulin, human recombinant (Life Technologies cat# 12585-014), 50 mg
Sodium Selenite (Sigma cat# S9133), 0.04326 mg
Β-Estradiol (Sigma cat# E2257), 0.02724 mg
L-Glutamine (ATCC 30-2214), 2.922 g
RPMI-1640 (ATCC 30-2001), 10 L

Adjust pH to 7.2 – 7.4. Filter sterilize through a 0.2 micrometer filter, dispense 500 mL into 500 mL bottles.

Note: Complete culture medium is stable for 1 month once hydrocortisone and transferrin are added.

Subculturing

Cultures can be maintained by addition of fresh medium once or twice a week.

Cryopreservation

liquid nitrogen vapor phase

Culture Conditions

Atmosphere: air, 95%; carbon dioxide (CO₂), 5%

Temperature: 37°C

Cells per Vial

Since being aggregates we use 20 ml culture medium with healthy aggregates per 1 ml freeze volume

Volume

1.0 mL

STR Profile

Amelogenin: X,Y

CSF1PO: 10,11

D13S317: 12

D16S539: 11

D5S818: 11

D7S820: 8,10

THO1: 9

TPOX: 8,11

vWA: 15,17

Sterility Tests

Bacteria and yeast: No growth
Mycoplasma: No growth

Viability

≥ 50%

Population Doubling Time

Approximately 24 hours per depositor

Name of Depositor

J Lee

Year of Origin

2014

References

Lee JK, et al. N-Myc drives neuroendocrine prostate cancer initiated from human prostate epithelial cells. Canc Cell 29(4):536-47, 2016. PubMed: 27050099

王經理

手機號：17280875617

QQ：1438578920
胡經理

手機號：13345964880

QQ：2438244627
周經理

手機號：17757487661

QQ：1296385441
于經理

手機號：18067160830

QQ：2088210172
沈經理

手機號：19548299266

QQ：2662369050
李經理

手機號：13626845108

QQ：972239479