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EL4-Luc2

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價格:

編號:B161693

品牌:Mingzhoubio

標準菌株

定量菌液

DNA

RNA

規格:

凍干粉

斜面

甘油

平板

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產品名稱

EL4-Luc2

商品貨號

B161693

Organism

Mus musculus, mouse

Tissue

blood

培養基

Product Format

frozen 1.0 mL

Morphology

lymphoblast

Culture Properties

suspension

Biosafety Level

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease

lymphoma

Strain

C57BL/6N

Applications

Excellent signal/background ratio and stable luciferase expression make this cell line ideal for in vivo bioluminescence imaging of xenograft animal model to study human cancer and monitor activity of anti-cancer drugs. It also can be used in cell-based assays for cancer research.

Comments

This luciferase expressing cell line was derived from parental line (ATCC TIB-39) by transduction with lentiviral vector encoding firefly luciferase gene (luc2) under control of EF-1 alpha promoter. This cell line was established through single cell cloning, and the cells constitutively express high levels of enzymatically active luciferase protein, which can be detected via in vitro and in vivo bioluminescence assays. The cells should be maintained in blasticidin (4 μg/mL) containing medium in routine cell culture. It is recommended to remove blasticidin prior to and during the experiment procedure when the cells are injected into animals in vivo, or co-cultured with other cell types in vitro.

Complete Growth Medium

The base medium for this cell line is ATCC-formulated Modified Eagle's Medium (DMEM, ATCC 30-2001). To make the complete growth medium, add the following components to the base medium:

Horse serum (FBS; ATCC 30-2040) to a final concentration of 10%
Blasticidin to a final concentration of 4 μg/mL

Subculturing

Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 x 10⁵ to 4.0 x 10 ⁵ cells/mL and maintain between 1 x 10⁵ and 1 x 10⁶ cells/mL.

Medium Renewal: every 2 to 3 days (depending on cell density).

Cryopreservation

Complete growth medium supplemented with 5% (v/v) DMSO

Culture Conditions

Atmosphere: air, 95%; carbon dioxide (CO₂), 5%

Temperature: 37°C

Cells per Vial

≥ 1.0 x 10⁶ cells

Volume

1.0 mL

Sterility Tests

Bacteria and yeast: No growth
Mycoplasma: No growth

Functional Tests

Luciferase activity: signal to noise ≥ 1,000 RLUs
In Vitro Luminesence: 20,000 photons/cell/sec, subject to imaging and culturing conditions

Population Doubling Time

approximately 19 hrs

Name of Depositor

ATCC

Year of Origin

2018

References

Zinn KR, et al. Noninvasive bioluminescence imaging in small animals. ILARJ 49: 103-115, 2008. PubMed: 18172337

Dothager RS, et al. Advances in bioluminescence imaging of live animal models. Curr Opin Biotechnol 20: 45-53, 2009. PubMed: 19233638

Ralph P. Retention of lymphocyte characteristics by myelomas and theta+-lymphomas: sensitivity to cortisol and phytohemagglutinin. J. Immunol. 110: 1470-1475, 1973. PubMed: 4541304

Old LJ, et al. The G (Gross) leukemia antigen. Cancer Res. 25: 813-819, 1965. PubMed: 4284252

Gorer PA. Studies in antibody response of mice to tumour inoculation. Br. J. Cancer 4: 372-379, 1950. PubMed: 14801344

Herberman RB. Serological analysis of cell surface antigens of tumors induced by murine leukemia virus. J. Natl. Cancer Inst. 48: 265-271, 1972. PubMed: 4119883

Ralph P, Nakoinz I. Inhibitory effects of lectins and lymphocyte mitogens on murine lymphomas and myelomas. J. Natl. Cancer Inst. 51: 883-890, 1973. PubMed: 4542714

Shevach EM, et al. Immunoglobulin and theta-bearing murine leukemias and lymphomas. J. Immunol. 108: 1146-1151, 1972. PubMed: 4112916

Aparicio CL, et al. Correction for label leakage in fluorimetric assays of cell adhesion. BioTechniques 23: 1056-1060, 1997. PubMed: 9421636

Cuthbert JA, Lipsky PE. Regulation of proliferation and Ras localization in transformed cells by products of mevalonate metabolism. Cancer Res. 57: 3498-3504, 1997. PubMed: 9270019

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