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pGC24 plasmid in E. coli

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編號:B161729

品牌:Mingzhoubio

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產(chǎn)品名稱

pGC24 plasmid in E. coli

商品貨號

B161729

Designations

pGC24 plasmid in E. coli

Species

Aspergillus awamori Nakazawa

培養(yǎng)基

Depositors

Cetus Corp., A Belt, Cetus Corp.

Applications

produces protein glucoamylase

Vector

Construct size (kb): 6.55

DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pTRP3
Intact vector size: 4.460
Type of vector: plasmid
Vector end: HindIII
Vector end: HindIII
Cloning sites: HindIII
Polylinker sites:
Other unique sites: BamHI
Construction: pVH153, pBR322
Host range: Escherichia coli
Features (with orientation and position when available):
promoter: trp, ->
ribosome-binding site: , ->
restriction site: HindIII, ->
marker(s): tetR, ->
replicon: pMB1
marker(s): ampR, <-
Cross references:

Insert

DNA: cDNA

DESCRIPTION OF INSERT COMPONENT:
Genome: Aspergillus awamori
Gene name: glucoamylase
Contains complete coding sequence?: U
Strain: NRRL 15271
Type of DNA: cDNA
Insert end: HindIII
Insert end: HindIII
Insert size (kb): 2.11
Cross references:

Insert lengths(kb): 2.10

Gene product: glucoamylase

Target Gene: glucoamylase

Insert Size (kb)

2.110

Media

ATCC^® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin

Biosafety Level

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Comments

Insert contains internal PstI and BamHI sites.

Restriction digests of the clone give the following sizes (kb): HindIII--4.5, 2.4; PstI--5.2, 1.9; BamHI--5.8, 1.1.

Expresses the gene product under the control of the Escherichia coli trp promoter.

The vector was constructed by inserting an EcoRI/ClaI fragment with Escherichia coli trp promoter and RBS into pBR322. A HindIII fragment of about 2.2 kb containing the gene with poly(A) signal was cloned into the HindIII site.

References

Nunberg J, et al. Glucoamylase cDNA. US Patent 4,794,175 dated Dec 27 1988

Disclosure

This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC.

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