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311-3D4
311-3D4
規格:
價格:
編號:B161864
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
311-3D4
商品貨號
B161864
Organism
Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Cell Type
hybridoma: B lymphocyte
培養基
Product Format
frozen
Morphology
lymphoblast
Culture Properties
suspension, but many cells attach
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Applications
Animals were immunized with a synthetic polypeptide with the sequence LWEIATYGMSPYPGIDLSQVY(C) corresponding to peptides 429 to 449 of the abl oncogene (formerly 548 to 568 of the v-abl oncogene).
In ELISA testing, the antibody reacts with the abl sequence.
No binding activity was detected for the homologous region of FES, v-erb B, v-src, SRC, v-yes, RAF-1, v-raf, fms, v-fps, v-ros, HIR, v-mos, and SYN.
In immunoblot testing a protein of approximately 40 kd is detected in ANN-1 and src transformed cells.
Derivation
Spleen cells were fused with Sp2/0 myeloma cells.
Genes Expressed
immunoglobulin; monoclonal antibody; against a synthetic abl oncogene peptide
Cellular Products
immunoglobulin; monoclonal antibody; against a synthetic abl oncogene peptide
Comments
Animals were immunized with a synthetic polypeptide with the sequence LWEIATYGMSPYPGIDLSQVY(C) corresponding to peptides 429 to 449 of the abl oncogene (formerly 548 to 568 of the v-abl oncogene).
Spleen cells were fused with Sp2/0 myeloma cells.
In ELISA testing, the antibody reacts with the abl sequence. No binding activity was detected for the homologous region of FES, v-erb B, v-src, SRC, v-yes, RAF-1, v-raf, fms, v-fps, v-ros, HIR, v-mos, and SYN.
In immunoblot testing a protein of approximately 40 kd is detected in ANN-1 and src transformed cells.
The IgG1 reactivity of the antibody with the ABL peptide (429 to 449) was confirmed by ELISA. IgG1 was strongly positive while IgG2b was negative.
Complete Growth Medium
The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density)
Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 2 X 10 exp5 viable cells/ml.
Maintain cell density between 2 X 10 exp5 and 1 X 10 exp6 viable cells/ml.
Scrape off the attached cells and transfer along with the floating cells into new flasks.
Cryopreservation
culture medium 95%; DMSO, 5%
Culture Conditions
Temperature: 37.0°C
Isotype
IgG1 and IgG2b
Antigenic Determinant
abl oncogene peptide, synthetic
Name of Depositor
National Cancer Institute
Deposited As
mouse (B cell); mouse (myeloma)
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