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p413 GPD

規(guī)格:

價(jià)格:

編號(hào):B161977

品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株

定量菌液

DNA

RNA

規(guī)格:

凍干粉

斜面

甘油

平板

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產(chǎn)品名稱

p413 GPD

商品貨號(hào)

B161977

Designations

p413 GPD

Depositors

M Funk

培養(yǎng)基

Biosafety Level

Host

Escherichia coli HB101 (ATCC 33694)

Vector Information

Intact vector size: 5.786
Type of vector: plasmid
Cloning sites: SalI ClaI EcoRV EcoRI SmaI BamHI SpeI XbaI
Polylinker sites: XhoI SalI ClaI HindIII EcoRV EcoRI PstI SmaI BamHI SpeI XbaI
Other unique sites: SacI
Construction: pRS413
Host range: Saccharomyces cerevisiae, Candida robusta; Escherichia coli

Features (with orientation and position when available):
marker(s): HIS3, →, 200-1395
terminator: CYC1, →, 2077-2337
MCS: SalI...XbaI, →, 2343-2401
promoter for expression: GPD, ←, 2401-3081
marker(s): ampR, ←, 4328-5258
replicon: CEN6/ARSH4, →, 5363-5876

Vector: p413 GPD (plasmid)

Promoters: Promoter for expression GPD

Construction: pRS413

Marker(s): ampR, HIS3

Construct size (kb): 5.851

Features: marker(s): HIS3
marker(s): ampR
promoter for expression: GPD
replicon: CEN6/ARSH4
MCS: SalI...XbaI
terminator: CYC1

Applications

YX-type (expression) shuttle vector

vector with low copy number

Comments

Restriction digests of the clone give the following sizes (kb): SacI/XbaI--5.2, 0.7; EcoRI--5.9; BamHI--5.9.

Low copy number shuttle expression vector.

Media

ATCC^® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin

Growth Conditions

Temperature: 37°C

References

Mumberg D, et al. Yeast vectors for the controlled expression of heterologous proteins in different genetic backgrounds. Gene 156: 119-122, 1995. PubMed: 7737504