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pY3

規格:

價格:

編號:B162203

品牌:Mingzhoubio

標準菌株

定量菌液

DNA

RNA

規格:

凍干粉

斜面

甘油

平板

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產品名稱

pY3

商品貨號

B162203

Designations

pY3

Depositors

K Blochlinger

培養基

Biosafety Level

Vector Information

Size (kb): 5.8699998855590820

Vector: pY3 (plasmid)

Promoters: Promoter Mo-SV LTR

Construction: pLG88, pMLTR-1

Marker(s):tetR,hygR

Construct size (kb): 5.869999885559082

Features: marker(s): hygR, tetR
promoter: Mo-SV LTR
replicon: pMB1
enhancer: Mo-MuLV LTR

Applications

shuttle vector

Comments

Restriction digests of the clone give the following sizes (kb): EcoRI--4.9, 0.9; KpnI--6.0; PstI--4.8, 1.2.

Plasmid vector allowing for direct selection for hygromycin B resistance after transfection of eukaryotic cell lines.

Media

ATCC^® Medium 1273: LB medium (ATCC medium 1065) with 20 mcg/ml tetracycline

Growth Conditions

Temperature: 37.0°C

References

Blochlinger K, Diggelmann H. Hygromycin B phosphotransferase as a selectable marker for DNA transfer experiments with higher eucaryotic cells. Mol. Cell. Biol. 4: 2929-2931, 1984. PubMed: 6098829

Shipped

freeze-dried

Shipping Information

Distributed: freeze-dried

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