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VIII-6G10
VIII-6G10
規格:
價格:
編號:B162279
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
VIII-6G10
商品貨號
B162279
Organism
Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Cell Type
hybridoma: B lymphocyte
培養基
Product Format
frozen
Morphology
lymphoblast
Culture Properties
suspension
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Applications
The antibody is specific for VCAM-1 and blocks up to 90% of lymphocyte adhesion to microvascular endothelial cells.
Disclosure
This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC.
Derivation
Animals were immunized with IL-4 and IL-1 beta activated endothelial cells isolated from mesenteric lymph nodes of Macaca nemestrina.
Spleen cells were fused with NS-1 myeloma cells.
Genes Expressed
immunoglobulin; monoclonal antibody; against vascular cell adhesion molecule 1 (VCAM-1, VCAM 1) of human and macaque
Cellular Products
immunoglobulin; monoclonal antibody; against vascular cell adhesion molecule 1 (VCAM-1, VCAM 1) of human and macaque
Tumorigenic
Yes
Effects
Yes, forms ascites in BALB/c mice
Comments
Animals were immunized with IL-4 and IL-1 beta activated endothelial cells isolated from mesenteric lymph nodes of Macaca nemestrina.
Spleen cells were fused with NS-1 myeloma cells.
The antibody is specific for VCAM-1 and blocks up to 90% of lymphocyte adhesion to microvascular endothelial cells.
Complete Growth Medium
RPMI 1640 medium with 25 mM HEPES, 2 mM L-glutamine and 1 mM sodium pyruvate, 90%; fetal bovine serum, 10%
Subculturing
Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
Cryopreservation
Culture medium, 95%; DMSO, 5%
Culture Conditions
Temperature: 37.0°C
Isotype
IgG1; kappa light chain
Name of Depositor
Fred Hutchinson Cancer Res. Cntr.
Deposited As
mouse (B cell); mouse (myeloma)
U.S. Patent Number
References

Masinovsky B, et al. IL-4 and TNF induce mAb 6G10-recognized expression on bone marrow stromal cells. US Patent 5,206,345 dated Apr 27 1993

Masinovsky B, et al. IL-4 acts synergistically with IL-1 beta to promote lymphocyte adhesion to microvascular endothelium by induction of vascular cell adhesion molecule-1. J. Immunol. 145: 2886-2895, 1990. PubMed: 1698865

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