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p559

規格:

價格:

編號:B162505

品牌:Mingzhoubio

標準菌株

定量菌液

DNA

RNA

規格:

凍干粉

斜面

甘油

平板

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產品名稱

p559

商品貨號

B162505

Designations

p559

Species

Homo sapiens, human

培養基

Vector

Construct size (kb): 4.400000095367432

Insert

DNA: cDNA

Insert lengths(kb): 1.399999976158142

Tissue: retina

Gene product: choroideremia(REP-1, Rab escort protein 1, tapeto-choroidal dystrophy, progressive choroidemia) [CHM]

Insert Size (kb)

1.400

Biosafety Level

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information

Distributed: DNA (dried). Rehydrate with TE. (amount: 200 ng)

Comments

Restriction digests of the clone give the following sizes (kb): EcoRI/HindIII--2.8, 0.80, 0.70; BamHI--4.4; BglII--4.4; EcoRI--3.5, 0.86; HindIII--4.4.

The insert contains the following restriction sites (approximate kb from the 5' end): PstI--0.28; BglII--0.47, 0.5; EcoRI--0.75; AccI--1.12. The insert is not cut with HindIII, BamHI, XbaI, SalI, or HincII.

Detects 9 exons contained in human genomic EcoRI fragments of the following sizes (kb, 5' to 3'): 7.5 (contains 3 exons), 4.5 or 4.2, 12.5, 2.6, 4.0, 1.0, 7.0.

Corresponds to the 5' EcoRI/NcoI fragment of T8.

Transcription from the SP6 and T7 promoters give the sense and antisense strands, respectively.

References

Cremers FP, et al. Cloning of a gene that is rearranged in patients with chroideraemia. Nature 347: 674-677, 1990. PubMed: 2215697

Frans P Cremers, personal communication

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