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p-rPrl
p-rPrl
規(guī)格:
貨期:
編號(hào):B162687
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱
p-rPrl
商品貨號(hào)
B162687
Designations
p-rPrl
Species
Rattus norvegicus, rat
Depositors
University of California, Davis, KH Murashige, University of California, Davis
Applications
For growth of the host strain, the medium must contain DAP (diaminopimelic acid) (100 mg/L) and thymidine (20 mg/L).
Vector
Construct size (kb): 5.190000057220459
DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pBR322
Intact vector size: 4.363
Type of vector: plasmid
Vector end: PstI
Vector end: PstI
Cloning sites: EcoRI ClaI HindIII EcoRV BamHI SphI SalI XmaIII NruI BspMI
BsmI StyI AvaI BalI BspMII PvuII Tth111I NdeI AflIII PpaI
PstI PvuI ScaI SspI AatII
Polylinker sites:
Construction: pBR313
Host range: Escherichia coli
Features (with orientation and position when available):
marker(s): tetR
replicon: pMB1
marker(s): ampR
Cross references: DNA Seq. Acc.: J01749
DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pBR322
Intact vector size: 4.363
Type of vector: plasmid
Vector end: PstI
Vector end: PstI
Cloning sites: EcoRI ClaI HindIII EcoRV BamHI SphI SalI XmaIII NruI BspMI
BsmI StyI AvaI BalI BspMII PvuII Tth111I NdeI AflIII PpaI
PstI PvuI ScaI SspI AatII
Polylinker sites:
Construction: pBR313
Host range: Escherichia coli
Features (with orientation and position when available):
marker(s): tetR
replicon: pMB1
marker(s): ampR
Cross references: DNA Seq. Acc.: J01749
Insert
DNA: cDNA
DESCRIPTION OF INSERT COMPONENT:
Genome: rat
Gene symbol: PRL
Gene name: prolactin
Contains complete coding sequence?: U
Chromosome: 17; Localization: 17
Tissue: anterior pituitary
Type of DNA: cDNA
Insert end: Modification: GC tails
Insert end: Modification: GC tails
Insert size (kb): 0.823
Cross references:
DESCRIPTION OF INSERT COMPONENT:
Genome: rat
Gene symbol: PRL
Gene name: prolactin
Contains complete coding sequence?: U
Chromosome: 17; Localization: 17
Tissue: anterior pituitary
Type of DNA: cDNA
Insert end: Modification: GC tails
Insert end: Modification: GC tails
Insert size (kb): 0.823
Cross references:
Insert lengths(kb): 0.8230000138282776
Tissue: anterior pituitary
Gene product: prolactin [PRL]
Insert Size (kb)
0.823
Media
ATCC® Medium 1083: LB medium for chi 1776
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information
Distributed: freeze-dried
Comments
Restriction digests of the clone give the following sizes (kb): HindIII--5.8; PvuII/PstI--3.0, 1.6, 0.9.
For growth of the host strain, the medium must contain DAP (diaminopimelic acid) (100 mg/L) and thymidine (20 mg/L).
The insert includes the complete open reading frame plus 5' and 3' untranslated regions.
References

Cooke NE, et al. Human and rat prolactin and preprolactin cloned genes. US Patent 4,725,549 dated Feb 16 1988

Disclosure
This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC.
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