最新中文字幕-精品国产99-国产精品福利在线观看-粉嫩av一区二区三区-日韩精品久久久久-美丽的姑娘国语视频-亚洲欧洲色-国产不卡毛片-三级黄毛片-免费福利在线-日韩av三区-欧美成年人网站-欧洲美女与动交ccoo-成人涩涩软件-秋霞影院午夜老牛影院-婷婷久久伊人-呦呦视频在线观看-www.天天综合-日韩精品高清视频-国产欧美综合在线-欧美日韩一区二区三区在线视频-a猛片免费播放-91九色蝌蚪国产-国产欧美日韩专区-嫩模被强到高潮呻吟不断

歷史搜索:A549    293T 金黃色葡萄球菌 大腸桿菌 AKK菌
購物車 1 種商品 - 共0元
當前位置: 首頁 > ATCC代理 > pMAP517 [112-12D] B162712
pMAP517 [112-12D]
pMAP517 [112-12D]
規格:
價格:
編號:B162712
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
pMAP517 [112-12D]
商品貨號
B162712
Designations
pMAP517 [112-12D]
Species
Bacillus thuringiensis subsp. kurstaki de Barjac and Lemille
培養基
Depositors
Monsanto Co., FJ Perlak, Monsanto Co.
Applications
produces protein crystal protein (delta endotoxin)
Vector
DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pMAP515
Intact vector size:
Type of vector: plasmid
Vector end: BglII
Vector end: BglII
Cloning sites: BglII
Polylinker sites:
Other unique sites: XhoI
Construction: pSUP1011, pMON7068, pMAP33
Features (with orientation and position when available):
Insert
DNA: cDNA
DESCRIPTION OF INSERT COMPONENT:
Genome: Bacillus thuringiensis subsp. kurstaki
Gene name: crystal protein (delta endotoxin)
Contains complete coding sequence?: Y
Strain: HD-1
Type of DNA: plasmid
Insert 5' end: BamHI
Insert 3' end: BamHI Modification: BamHI -> BglII
Insert size (kb): 4.6
Cross references:
Insert lengths(kb): 4.599999904632568
Gene product: crystal protein (delta endotoxin)
Target Gene: crystal protein (delta endotoxin)
Insert Size (kb)
4.600
Media
ATCC® Medium 1236: LB Medium (ATCC medium 1065) with 25 mcg/ml kanamycin
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information
Distributed: freeze-dried
Comments
The IS50L(kanR/B.t.) gene segment was integrated into the host chromosome by mating E. coli S17-1 containing pMAP517 with P. fluorescens 112-12.
The IS50L(kanR/B.t.) gene segment has a +4 frameshift mutation (which converts a wild type XhoI site to a PvuI site) of the transposase gene IS50L.
This strain is resistant to 100 mcg/ml rifampicin and 100 mcg/ml nalidixic acid.
Strain that proliferates in a plant environment to an extent sufficient to express at least 0.5 ng of insecticidally active protein per each insect to be controlled.
References

Obukowicz MG, et al. Combatting plant insect pests with plant-colonizing microorganisms containing the toxin gene B. thuringiensis as a chromosomal insertion. US Patent 5,229,112 dated Jul 20 1993

Disclosure
This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC.
  • 王經理
    手機號：17280875617
    QQ：1438578920
  • 胡經理
    手機號：13345964880
    QQ：2438244627
  • 周經理
    手機號：17757487661
    QQ：1296385441
  • 于經理
    手機號：18067160830
    QQ：2088210172
  • 沈經理
    手機號：19548299266
    QQ：2662369050
  • 李經理
    手機號：13626845108
    QQ：972239479