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Human herpesvirus 5

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貨期:

編號:B162785

品牌:Mingzhoubio

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定量菌液

DNA

RNA

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產品名稱

Human herpesvirus 5

商品貨號

B162785

Classification

Herpesviridae, Cytomegalovirus

Agent

Human herpesvirus 5

Strain

Merlin

Common Name

Human cytomegalovirus

Biosafety Level

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Product Format

frozen 1 mL per vial

Storage Conditions

-70°C or colder

Genome Sequenced Strain

Human herpesvirus 5 strain Merlin, complete genome

Comments

Low MOI applications, such as titration, may require incubation for as long as 3 weeks. This is a low passage strain of Human herpesvirus 5, deposited at ATCC^® 4 passages from isolation. At deposit, it retained the wild type complement of 165 genes, and contained no obvious mutations other than a single nucleotide substitution that truncates gene UL128. To retain genetic integrity, further passage should be limited.

Effect on Host

CPE, cell rounding and enlargement, cytoplasmic and nuclear inclusions

Recommended Host

MRC-5 (ATCC^® CCL-171™)

Growth Conditions

Temperature: 37°C
Recommendations for Infection: For best results, infection should be performed on a 80-90% confluent, 18-48 hour old cellular monolayer. Prepare dilution of virus in minimum amount of volume (e.g. 1 mL per 25 cm²). Wash monolayer with PBS or serum free medium prior to inoculation. Adsorb virus dilution for 1-2 hours at 37°C in a humidified 5% CO₂ atmosphere, rocking every 20-30 minutes to redistribute inoculum. End adsorption by adding virus growth medium.
Incubation: 2-10 days at 37°C in a humidified 5% CO₂ atmosphere, until CPE is progressed through 80% of the monolayer.

Name of Depositor

GWG Wilkinson, Cardiff Public Health Laboratory

Source

Urine from a congenitally infected infant, Cardiff, Wales

References

Davison A J, et al. Homology between the human cytomegalovirus RL11 gene family and human adenovirus E3 genes. J. Gen. Virol. 84 (Pt 3), 657-663 (2003). PubMed: 12604818

Dolan A, et al. Genetic content of wild-type human cytomegalovirus. Journal of General Virology, 85: 1301-1312, 2004. PubMed: 15105547

Cross References

Nucleotide (GenBank) : AY446894.2 Human herpesvirus 5 strain Merlin, complete genome

B244801：Human adenovirus 21

B244635：Human echovirus 31

B244601：Human herpesvirus 5

B244599：DNA from Human herpesvirus 1, strain HF

B244392：Human echovirus 21

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