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Porcine rotavirus
Porcine rotavirus
規格:
貨期:
編號:B162786
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
Porcine rotavirus
商品貨號
B162786
Classification
Reoviridae, Rotavirus
Agent
Porcine rotavirus
Strain
OSU (attenuated)
Applications
Serotype 5 (G5) by antigenic typing at VP7 and serotype P7 by antigenic typing at VP4. Preliminary trials in gnotobiotic pigs indicate that this preparation is attenuated.
Biosafety Level
2

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Isolation
clinical specimen - animal
Ohio, United States
Product Format
frozen
Storage Conditions
-70°C or colder (or colder)
Comments
Serotype 5 (G5) by antigenic typing at VP7 and serotype P7 by antigenic typing at VP4. Preliminary trials in gnotobiotic pigs indicate that this preparation is attenuated.
Effect on Host
Yes, in vitro effects: Cytopathic effects, some initial rounding followed by degeneration of cells and eventual sloughing
CPE, some initial rounding followed by ????? degeneration of cells and eventual sloughing
Growth Medium
Minimum essential medium (Eagle) with 2 mM L-glutamine and Earle?s BSS adjusted to contain 1.5 g/L sodium bicarbonate, 0.1 mM non-essential amino acids, 1.0 mM sodium pyruvate, and 0.008 mg/ml trypsin (GIBCO 27250-018). No serum is used.
Recommended Host

Recommended Host: MA-104 cells (ATCC CRL-

????? 2378.1)

Growth Conditions
Temperature: 37.0°C
Duration: 1-4 days; 5% CO2 in air atmosphere is recommended; For best results cells should be 24 to 48 hours old and 90-100% confluent.
Protocol: This rotavirus should be grown in the presence of trypsin or pancreatin and NO serum should be used. Preliminary trials in gnotobiotic pigs indicate that this preparation is attenuated, but no back passages have been done. Cross-reacts with group A rotaviruses from other species. Heat labile (56°C, 30min.). Stable at pH 3.0 and to ether.
Cryopreservation
Storage temperature: -70°C
Subcultivation
Protocol: This rotavirus should be grown in the presence of trypsin or pancreatin and NO serum should be used. Preliminary trials in gnotobiotic pigs indicate that this preparation is attenuated, but no back passages have been done. Cross-reacts with group A rotaviruses from other species. Heat labile (56°C, 30min.). Stable at pH 3.0 and to ether.
Name of Depositor
LJ Saif
Source
clinical specimen - animal
Ohio, United States
References

Saif LJ, et al. Immune electron microscopy of transmissible gastroenteritis virus and rotavirus (reovirus-like agent) of swine. Am. J. Vet. Res. 38: 13-20, 1977. PubMed: 189646

Theil KW, et al. Pathogenesis of porcine rotaviral infection in experimentally inoculated gnotobiotic pigs. Am. J. Vet. Res. 39: 213-220, 1978. PubMed: 204232

Rosen BI, et al. Characterization of full-length and polymerase chain reaction-derived partial-length Gottfried and OSU gene 4 probes for serotypic differentiation of porcine rotaviruses. J. Clin. Microbiol. 30: 2644-2652, 1992. PubMed: 1328281

Theil KW, et al. Cell culture propagation of porcine rotavirus (reovirus-like agent). Am. J. Vet. Res. 38: 1765-1768, 1977. PubMed: 201198

or colder

intestinal contents of one- to four-week-old pigs with diarrhea

or colder

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