Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Rickettsia monacensis, strain IrR/Munic, complete genome.
Temperature: 34°C
Recommendations for Infection: Plate cells 18-24 hours prior to infection and infect when cultures are 65-75% confluent. Remove DMSO from bacteria vial by centrifugation (180 x g for 10 minutes at 4°C). Remove supernatant and add 2 mL of fresh medium to the pellet. Pulse vortex for 1 minute. Remove medium from host cell culture and inoculate with bacteria (e.g. 1 mL per 25 cm2) diluted to provide an optimal MOI (e.g. 0.1 to 1). Adsorb by centrifugation for 30 minutes at 800 x g at 22°C. Add agent growth medium following adsorption.
Incubation: 6-11 days at 34°C in a humidified atmosphere, until CPE is progressed through 80% of the monolayer. A Giemsa stain may also be used to determine end of incubation.
Simser JA, et al. Rickettsia monacensis sp. nov., a spotted fever group Rickettsia, from ticks (Ixodes Ricinus) collected in a European city park. Appl Environ Microbiol 68(9): 4559-4566, 2002. PubMed: 12200314
Nucleotide (GenBank) : NZ_LN794217.1 Rickettsia monacensis, strain IrR/Munic, complete genome.
