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Human coronavirus 229E
Human coronavirus 229E
規(guī)格:
價(jià)格:詢價(jià)
編號(hào):B163009
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱(chēng)
Human coronavirus 229E
商品貨號(hào)
B163009
Classification
Coronaviridae, Coronavirus
Agent
Human coronavirus 229E
培養(yǎng)基
Strain
229E
Common Name
Human coronavirus
Applications
No hemolysis production.
Respiratory research
Biosafety Level
2

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Product Format
frozen 1 mL per vial
Storage Conditions
-70°C or colder
Comments
Possibility of cross-reactions with other coronaviruses not thoroughly characterized at time of deposit (1973); does not cross-react with human myxoviruses. Ether sensitive. No hemolysis production. Unstable at 24 & 37C; relatively stable at 10C.
Harvest 26-50 hrs post adsorption before CPE is visible. Does not agglutinate chicken, guinea pig, human O, sheep, mouse or rat RBC. Size 89Mu (gradocol filtration). [RU1 are human embryonic lung fibroblasts developed by CDC]
Effect on Host
CPE, rounding and sloughing. Starts as individual cell rounding that spreads, but over time uninfected cells repopulate monolayer.
Recommended Host
MRC-5 (ATCC® CCL-171™)
Growth Conditions
Temperature: 35°C
Recommendations for Infection: For best results cells should be 24 to 48 hours old and 80-90% confluent (not 100% confluent).
Incubation: 2-7 days, a 5% CO2 in air atmosphere is recommended.
Name of Depositor
HS Kaye
Special Collection
ATCC
Source
Nasal and throat swabs from man with mild upper respiratory illness
References

Hamre D, Procknow JJ. A new virus isolated from the human respiratory tract. Proc. Soc. Exp. Biol. Med. 121: 190-193, 1966. PubMed: 4285768

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