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Vaccinia virus ts mutant
Vaccinia virus ts mutant
規格:
貨期:
編號:B163050
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
Vaccinia virus ts mutant
商品貨號
B163050
Classification
Poxviridae, Orthopoxvirus
Agent
Vaccinia virus ts mutant
Strain
IHD-W Dts86
Applications
The mutation was assigned by complementation analysis (Lackner, et al, 2003) to vaccinia map location A24, the 132-kDa RNA polymerase subunit.
Vaccination is recommended for laboratory staff manipulating vaccinia, monkeypox, and cowpox viruses.
Biosafety Level
2

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Isolation
This conditional lethal mutant, originally designated plaque isolate number 9179, was isolated from the IHD-W strain of Vaccinia by S. Dales. The IHD-W strain was derived from an IHD-J strain. This mutant was selected after mutagenesis with nitrosoguanidine and replication in the presence of bromodeoxyuridine. This mutant was not assigned to a Dales EM category. The mutation was assigned by complementation analysis (Lackner, et al, 2003) to vaccinia map location A24, the 132-kDa RNA polymerase subunit. The non-permissive incubation temperature is 39.5°C.
Product Format
frozen
Storage Conditions
-70°C or colder
Comments
This conditional lethal mutant, originally designated plaque isolate number 9179, was isolated from the IHD-W strain of Vaccinia by S. Dales. The IHD-W strain was derived from an IHD-J strain. This mutant was selected after mutagenesis with nitrosoguanidine and replication in the presence of bromodeoxyuridine. This mutant was not assigned to a Dales EM category. The mutation was assigned by complementation analysis (Lackner, et al, 2003) to vaccinia map location A24, the 132-kDa RNA polymerase subunit. The non-permissive incubation temperature is 39.5°C.
Vaccination is recommended for laboratory staff manipulating vaccinia, monkeypox, and cowpox viruses.
Effect on Host
CPE: enlargement, plaques with rounding and eventual sloughing
Cytopathic effects in culture include enlargement and rounding of cells, followed by death and sloughing.
Growth Medium
ATCC® Medium 30-2002: Dulbecco's Modified Eagle's Medium (DMEM)
Fetal Bovine Serum
Recommended Host

Recommended Host: ?BSC-40 (ATCC CRL-2761)

Production host: BSC-40 (ATCC CRL-2761) Alternate hosts: MRC-5, BSC-1, HeLa, LLC-MK2
Growth Conditions
For best results, plate cells 24-48 hours in advance in DMEM with 10% FBS at 37.0°C. Infect at 90-100% confluence using a multiplicity of infection (MOI) of about 1. Infect and incubate in DMEM supplemented with 2% FBS at 32.0°C for 1-3 days. Harvest cells and culture fluids when 100% of culture shows advanced cytopathic effects. Vaccinia virus is largely cell associated. Concentrated preparations can be made by collection and sonication of cell pellets.
Name of Depositor
S. Dales, R. Condit
References

Dales S, et al. Biogenesis of vaccinia: isolation of conditional lethal mutants and electron microscopic characterization of their phenotypically expressed defects. Virology 84: 403-428, 1978. PubMed: 622807

Lackner CA, et al. Complementation analysis of the Dales collection of vaccinia virus temperature-sensitive mutants. Virology 305: 240-259, 2003. PubMed: 12573570

Condit RC, et al. Isolation, characterization, and physical mapping of temperature-sensitive mutants of vaccinia virus. Virology 128: 429-443, 1983. PubMed: 6577746

Biosafety in Microbiological and Biomedical Laboratories, 5th ed. HHS. U.S. Department of Health and Human Services, Centers for Disease Control and Prevention. Washington DC: U.S. Government Printing Office; 2007. The entire text is available online.

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