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Simian adenovirus 7

規(guī)格:

價格:

編號:B163368

品牌:Mingzhoubio

標準菌株

定量菌液

DNA

RNA

規(guī)格:

凍干粉

斜面

甘油

平板

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產(chǎn)品名稱

Simian adenovirus 7

商品貨號

B163368

Classification

Adenoviridae, Mastadenovirus

Deposited As

SV25

培養(yǎng)基

Agent

Simian adenovirus 7

Strain

8045-2WN

Applications

Respiratory research

Biosafety Level

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Product Format

frozen

Storage Conditions

-70°C or colder

Comments

Simian Virus Group I (Hull).
REFERENCE STRAIN: Pereira, H.G. et al., Virology 20:613, 1963.
Originally deposited as SV25

Effect on Host

CPE, refractile rounding and sloughing

Recommended Host

LLC-MK₂ (ATCC CCL-7.1)

Growth Conditions

Temperature: 37°C
Recommendations for Infection: For best results, infection should be performed on a 80-90% confluent, 18-48 hour old cellular monolayer. Prepare dilution of virus in minimum amount of volume (e.g. 1 mL per 25 cm²). Wash monolayer with PBS or serum free medium prior to inoculation. Adsorb virus dilution for 1-2 hours at 37°C in a humidified 5% CO₂ atmosphere, rocking every 20-30 minutes to redistribute inoculum. End adsorption by adding virus growth medium.
Incubation: 5-14 days at 37°C in a humidified 5% CO₂ atmosphere, until CPE is progressed through 80% of the monolayer.

Name of Depositor

RN Hull

Source

Tissue cultures prepared from pooled rhesus monkey kidney cells

References

Minner JR, et al. New viral agents recovered from tissue cultures of monkey kidney cells. III. Recovery of additional agents both from cultures of monkey tissues and directly from tissues and excreta. Am. J. Hyg. 68: 31-44, 1958. PubMed: 13559209

Roy S, et al. Construction of gene transfer vectors based on simian adenovirus 7. J. Gen. Virol. 92(8): 1749-1753, 2011. PubMed: 21562118

Cross References

Nucleotide (GenBank) : DQ792570.1 Simian adenovirus 7, complete genome

B245003：pUCSVH388-2 [molecular clone of Simian virus 40, SV-40]

B244100：Simian foamyvirus type 5

B243389：Simian foamyvirus type 7

B242028：Simian virus 40

B241399：Simian adenovirus 18

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