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<em>Chlamydia trachomatis</em>

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編號:B163416

品牌:Mingzhoubio

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DNA

RNA

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產品名稱

Chlamydia trachomatis

商品貨號

B163416

Classification

Chlamydiaceae, Chlamydia

Deposited As

Lymphogranuloma venereum

培養基

Agent

Chlamydia trachomatis

Strain

Lymphogranuloma venereum (LGV I) strain 440

Applications

Sexually Transmitted Disease Research

Biosafety Level

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Product Format

frozen 1 mL per vial

Storage Conditions

-70°C or colder

Comments

FBS used to culture Chlamydia must be prescreened to verify that the serum does not contain antibodies to Chlamydia or other factors that would interfere with growth.

Centrifuge at 2000 X g for 1 hour; adsorb 1 hour at 35-36°C. Feed with 1.0 mL EMEM (HEPES buffered) 5mg glucose/mL, 0.3 mg glutamine/mL, 1-2 µg/mL cycloheximide. Dilute high titer stocks to avoid cell toxicity.

Tissue culture adapted at ATCC.

Next-generation sequencing (NGS) at ATCC on the McCoy cell line (ATCC^® CRL-1696™) used as the host has shown the presence of Mus Musculus mobilized endogenous polytropic provirus and Murine leukemia virus.

Effect on Host

Intracellular inclusion bodies visualized by fluorescent staining with genus or species specific conjugated antibodies.

Recommended Host

McCoy cells (ATCC^® CRL-1696™)

Growth Conditions

Temperature: 37°C

Recommendations For Infection: For best results cells should be 24 to 48 hours old and 80-90% confluent.

Incubation: 2-3 days, a 5% CO₂ in air atmosphere is recommended

Name of Depositor

J Schachter

Source

Lymph node from human with LGV

References

Schachter J, et al. Lymphogranuloma venereum. I. Comparison of the Frei test, complement fixation test, and isolation of the agent. J. Infect. Dis. 120: 372-375, 1969. PubMed: 5822618

Schachter J, Meyer KF. Lymphogranuloma venereum. II. Characterization of some recently isolated strains. J. Bacteriol. 99: 636-638, 1969. PubMed: 4905529

Cross References

Nucleotide (GenBank) : AJ012175 Chlamydia trachomatis partial rnpB gene for RNase P RNA subunit.

B244810：Chlamydia trachomatis

B241655：Chlamydia trachomatis Serovar F

B236874：Chlamydia trachomatis

B219385：Chlamydia trachomatis

B216943：DNA from Chlamydia trachomatis LGV Serovar I

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