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Bovine adenovirus 4
Bovine adenovirus 4
規(guī)格:
價(jià)格:
編號(hào):B163431
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱(chēng)
Bovine adenovirus 4
商品貨號(hào)
B163431
Classification
Adenoviridae, Atadenovirus,
Deposited As
Bovine adenovirus type 4
培養(yǎng)基
Agent
Bovine adenovirus 4
Strain
THT/62
Applications
Respiratory research
Biosafety Level
2

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Isolation
Lungs of a calf with pneumoenteritis
Storage Conditions
?-70°C or colder
Comments
Does not share common CF antigen with human adenoviruses. No cross-reactivity with bovine adenovirus types 1-3.
GenBank: AF036092.3
Effect on Host
CPE, generalized enlarged cell rounding and cell clumping or clustering; Type B intranuclear inclusion bodies
?CPE, generalized enlarged cell rounding and cell clumping or clustering; Type B intranuclear inclusion bodies
Recommended Host
BT (ATCC CRL-1390)

Production Host: ?BT (ATCC? CRL-1390™)

Growth Conditions
Temperature: 37.0°C
Recommendations for Infection: Plate cells 24-48 hours prior to infection and infect when cultures are 80-90% confluent. Remove medium and inoculate with a small volume (e.g. 1 mL per 25 cm2) of virus diluted to provide an optimal MOI (e.g. 0.1). Adsorb 1-2 hours at 37°C without CO2, rocking every 20-30 minutes to redistribute inoculum. End adsorption by adding virus growth medium.
Incubation: Incubate infected culture for 6-7 days at 37°C without CO2, until 90% of the culture shows CPE.
Name of Depositor
SB Mohanty
Chain of Custody
Deposited as Bovine adenovirus type 4. Originally received after 6 passages in primary calf testicular cells.
Source
Lungs of a calf with pneumoenteritis
References

Bartha A, Aldasy P. Further two serotypes of bovine adenoviruses (serotype 4 and 5). Acta Vet. Acad. Sci. Hung. 16(1): 107-108, 1966. PubMed: 5917816

Cross References

GenBank : AF036092.3 Bovine adenovirus 4 strain THT/62, complete genome.

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