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10B9
10B9
規(guī)格:
價格:
編號:B163536
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱
10B9
商品貨號
B163536
Organism
Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Cell Type
hybridoma: B lymphocyte
培養(yǎng)基
Product Format
frozen
Morphology
lymphoblast
Culture Properties
suspension
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Applications
The antibody reacts with human umbilical vein endothelial cells and the immature macrophage cell line, U937.
Tested and found negative for ectromelia virus (mousepox).
Derivation
Spleen cells were fused with P3X63Ag8.653 myeloma cells.
Genes Expressed
immunoglobulin; monoclonal antibody; against human endothelium
Cellular Products
immunoglobulin; monoclonal antibody; against human endothelium
Comments
Animals were immunized with human umbilical vein endothelial cells.
Spleen cells were fused with P3X63Ag8.653 myeloma cells.
The antibody reacts with human umbilical vein endothelial cells and the immature macrophage cell line, U937.
It does not react with bovine pulmonary or aortic endothelial cells, human monocytes or mature cultured macrophages or DHL-2 cells.
Tested and found negative for ectromelia virus (mousepox).
Complete Growth Medium
Culture Medium: RPMI medium 1640, 85%; fetal bovine serum, 15%.
Subculturing
Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
Isotype
IgG1; kappa light chain
Name of Depositor
YA Reid, AW Hamburger
Deposited As
mouse (B cell); mouse (myeloma)
References

Hamburger AW, et al. Isolation and characterization of monoclonal antibodies reactive with endothelial cells. Tissue Cell 17: 451-459, 1985. PubMed: 3901396

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