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10P12
10P12
規格:
價格:
編號:B163538
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
10P12
商品貨號
B163538
Organism
Mus musculus, mouse
Cell Type
mast cell; Abelson murine leukemia virus transformed
培養基
Product Format
frozen
Morphology
mast cell
Culture Properties
suspension
Biosafety Level
2

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Age
10 days gestation
Applications
The 10P12 cell line was established by transformation of placental cells from a 10 day mouse embryo with A-MuLV.
Storage Conditions
liquid nitrogen vapor phase
Derivation
The 10P12 cell line was established by transformation of placental cells from a 10 day mouse embryo with A-MuLV.
Receptor Expression
FcERI (Fc of IgE)
Comments
The 10P12 cell line was established by transformation of placental cells from a 10 day mouse embryo with A-MuLV.
The cells are surface immunoglobulin positive, B23.1 positive, B220 positive and possess receptors for IgE.
This line represents a transformed stage of a very early hematopoietic precursor and shares surface antigens with multipotent stem cells.
Transcripts of germline immunoglobulin and T cell receptor genes are detectable.
Complete Growth Medium
RPMI 1640 medium with 0.05 mM 2-mercaptoethanol, 90%; fetal bovine serum, 10%
Subculturing
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 x 104 cells/mL and maintain between 1 x 104 and 5 x 105 cells/mL. Do not allow the cell density to exceed 5 x 105 cells/mL.
Medium Renewal: Two to three times weekly
Cryopreservation

Complete growth medium described above supplemented with 5% (v/v) DMSO.  Cell culture tested DMSO is available as ATCC Catalog No. 4-X.

Culture Conditions
Temperature: 37°C
Atmosphere: Air, 95%; Carbon dioxide (CO2), 5%
Name of Depositor
EJ Siden
Deposited As
Mus musculus
References

Siegel ML, et al. Isolation and characterization of Abelson murine leukemia virus- transformed mast cell lines from midgestation embryonic placenta. Eur. J. Immunol. 15: 1136-1141, 1985. PubMed: 2998805

Siden EJ. Regulated expression of germline antigen receptor genes in mast cell lines from the murine embryo. J. Immunol. 150: 4427-4437, 1993. PubMed: 7683318

Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC.

Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988.

Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC.

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