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1116-NS-19-9
1116-NS-19-9
規格:
價格:
編號:B163539
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
1116-NS-19-9
商品貨號
B163539
Organism
Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Cell Type
hybridoma: B lymphocyte
培養基
Product Format
frozen
Morphology
lymphoblast
Culture Properties
suspension
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease
Carcinoma,Cancer
Applications
The antibody binds to human tumors of endodermal origin (gastric, pancreatic and colorectal carcinomas) and detects the antigen in serum and urine of cancer patients.
Disclosure
This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC.
Derivation
Spleen cells were fused with P3X63Ag8 myeloma cells.
Clinical Data
The antibody binds to human tumors of endodermal origin (gastric, pancreatic and colorectal carcinomas) and detects the antigen in serum and urine of cancer patients.
Genes Expressed
immunoglobulin; monoclonal antibody; against colorectal carcinoma monosialoganglioside
Cellular Products
immunoglobulin; monoclonal antibody; against colorectal carcinoma monosialoganglioside
Comments
Animals were immunized with the SW1116 human colon carcinoma cell line (ATCC CCL-233).
Spleen cells were fused with P3X63Ag8 myeloma cells.
The antibody binds to human tumors of endodermal origin (gastric, pancreatic and colorectal carcinomas) and detects the antigen in serum and urine of cancer patients.
Complete Growth Medium
The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 20%.
Subculturing
Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
Cryopreservation
Culture medium, 95%; DMSO, 5%
Isotype
IgG1
Name of Depositor
Wistar Institute
Deposited As
mouse (B cell); mouse (myeloma)
U.S. Patent Number
References

Steplewski Z, et al. Detection of colorectal carcinoma. US Patent 4,471,057 dated Sep 11 1984

Koprowski H, et al. Colorectal carcinoma antigens detected by hybridoma antibodies. Somatic Cell Genet. 5: 957-971, 1979. PubMed: 94699

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