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116 [Anti PDGF Hybridoma #116]
116 [Anti PDGF Hybridoma #116]
規格:
價格:
編號:B163543
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
116 [Anti PDGF Hybridoma #116]
商品貨號
B163543
Organism
Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Cell Type
hybridoma: B lymphocyte
培養基
Product Format
frozen
Morphology
lymphoblast
Culture Properties
suspension
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Applications
The antibody is specific for the B chain of PDGF v-sis.
Disclosure
This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC.
Derivation
Animals were immunized with affinity purified PDGF B v-sis.
Spleen cells were fused with Sp2/0-Ag14 myeloma cells.
Genes Expressed
immunoglobulin; monoclonal antibody; against platelet derived growth factor B chain (PDGF B, v-sis form)
Cellular Products
immunoglobulin; monoclonal antibody; against platelet derived growth factor B chain (PDGF B, v-sis form)
Tumorigenic
Yes
Effects
Yes, forms ascites in pristane primed BALB/c mice
Comments
Animals were immunized with affinity purified PDGF B v-sis.
Spleen cells were fused with Sp2/0-Ag14 myeloma cells.
The antibody is specific for the B chain of PDGF v-sis.
Complete Growth Medium
Dulbecco's modified Eagle's medium with 4.5 g/L glucose, 90%; heat-inactivated fetal bovine serum, 10%
Subculturing
Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
Cryopreservation
fetal bovine serum, 90%; DMSO, 10%
Isotype
IgG2a
Name of Depositor
Amgen, Inc.
Deposited As
mouse (B cell); mouse (myeloma)
U.S. Patent Number
References

Thomason AR, Nicolson MA. Methods for purification of platelet-derived growth factor. US Patent 5,175,255 dated Dec 29 1992

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