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11E10
11E10
規格:
價格:
編號:B163546
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
11E10
商品貨號
B163546
Organism
Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Cell Type
hybridoma: B lymphocyte
培養基
Product Format
frozen
Morphology
lymphoblast
Culture Properties
suspension
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Applications
Animals were immunized with a formalinized toxoid prepared from crude SLT-II produced by an E. coli K-12 strain lysogenized with the 933W toxin converting phage of enterohemorrhagic E. coli 933.
The antibody reacts with the A subunit of SLT-II and does not react with B subunit.
The antibody can be used in SLT-II neutralization assays and in assays to detect SLT-II producing E. coli strains.
It may also be used to differentiate SLT-II from SLT-I, SLT-IIv and Shiga toxin.
The 11E10 antibody differs from that of 11F11 (ATCC CRL-1908) in that it can partially neutralize SLT-IIv.
Derivation
Animals were immunized with a formalinized toxoid prepared from crude SLT-II produced by an E. coli K-12 strain lysogenized with the 933W toxin converting phage of enterohemorrhagic E. coli 933.
Spleen cells were fused with Sp2/0-Ag14 myeloma cells.
Genes Expressed
immunoglobulin; monoclonal antibody; against Shiga like toxin II (SLT-II)
Cellular Products
immunoglobulin; monoclonal antibody; against Shiga like toxin II (SLT-II)
Comments
Animals were immunized with a formalinized toxoid prepared from crude SLT-II produced by an E. coli K-12 strain lysogenized with the 933W toxin converting phage of enterohemorrhagic E. coli 933.
Spleen cells were fused with Sp2/0-Ag14 myeloma cells.
The antibody reacts with the A subunit of SLT-II and does not react with B subunit.
The antibody can be used in SLT-II neutralization assays and in assays to detect SLT-II producing E. coli strains.
It may also be used to differentiate SLT-II from SLT-I, SLT-IIv and Shiga toxin.
The 11E10 antibody differs from that of 11F11 (ATCC CRL-1908) in that it can partially neutralize SLT-IIv.
Complete Growth Medium
The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Medium Renewal: Every 2 to 4 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 1 x 10 exp5 cells/ml and maintain between 1 x 10 exp5 and 1 x 10 exp6 cells/ml.
Isotype
IgG1; kappa light chain
Name of Depositor
AD O'Brien
References

Perera LP, et al. Isolation and characterization of monoclonal antibodies to Shiga-like toxin II of enterohemorrhagic Escherichia coli and use of the monoclonal antibodies in a colony enzyme-linked immunosorbent assay. J. Clin. Microbiol. 26: 2127-2131, 1988. PubMed: 3053764

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