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12.1
12.1
規格:
價格:
編號:B163549
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
12.1
商品貨號
B163549
Organism
Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Cell Type
hybridoma: B lymphocyte
培養基
Product Format
frozen
Morphology
lymphoblast
Culture Properties
suspension
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease
sarcoma
Applications
The antibody strongly reacts with an antigen (CD6) expressed on peripheral blood T cells, activated T cells and Sezary type leukemic T cells.
Lesser amounts of the antigen were detected on thymocytes and acute T cell leukemic lympoblasts.
The antibody also reacts with leukemic cells from most patients with B cell chronic lymphocytic leukemia and some patients with lymphosarcoma cell leukemia.
Derivation
Animals were immunized with human lymphoblasts from a mixed lymphocyte culture reaction.
Spleen cells were fused with NSI/1 myeloma cells.
Genes Expressed
immunoglobulin; monoclonal antibody; against human CD6
Cellular Products
immunoglobulin; monoclonal antibody; against human CD6
Comments
The antibody strongly reacts with an antigen (CD6) expressed on peripheral blood T cells, activated T cells and Sezary type leukemic T cells.
Lesser amounts of the antigen were detected on thymocytes and acute T cell leukemic lympoblasts.
The antibody also reacts with leukemic cells from most patients with B cell chronic lymphocytic leukemia and some patients with lymphosarcoma cell leukemia.
Complete Growth Medium
The base medium for this cell line is RPMI 1640 Medium (ATCC 30-2001). To make the complete medium add the following components to 500 mL of the base medium: 
  • 2-mercaptoethanol for a final concentration of 50 µM
  • 56 mL Fetal Bovine Serum (FBS; ATCC 30-2020) for a final concentration of 10%
Subculturing
Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 1 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 5 X 10 exp5 cells/ml.
Isotype
IgG2a
Name of Depositor
PJ Martin
Deposited As
mouse (B cell); mouse (myeloma)
References

Kamoun M, et al. A novel human T cell antigen preferentially expressed on mature T cells and shared by both well and poorly differentiated B cell leukemias and lymphomas. J. Immunol. 127: 987-991, 1981. PubMed: 6790625

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