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132-1C8
132-1C8
規格:
價格:
編號:B163562
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
132-1C8
商品貨號
B163562
Organism
Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Tissue
spleen
培養基
Cell Type
hybridoma: B lymphocyte
Product Format
frozen
Morphology
lymphoblast
Culture Properties
suspension
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Age
adult
Gender
male
Strain
129GIX+
Applications
The antibody reacts with the synthetic MYB oncogene peptide.
In BM2 cells, proteins of 50 kd and 20 kd are detected.
In human tumor (MOLT 4), a 60 kd protein is detected as well as two proteins of 40 and 45 kd.
Animals were immunized with a synthetic polypeptide with the sequence RHYDEDPEKEKRIKELEL corresponding to peptides 282 to 300 of the MYB oncogene.
Storage Conditions
liquid nitrogen vapor phase
Derivation
Animals were immunized with a synthetic polypeptide with the sequence RHYDEDPEKEKRIKELEL corresponding to peptides 282 to 300 of the MYB oncogene. Spleen cells were fused with Sp2/0 myeloma cells. The antibody reacts with the synthetic MYB oncogene peptide. In immunoblot assays, the antibody recognizes proteins of approximately 80 kd and 60 kd in extracts from MSTF cells (mink lung cells transformed by fes). In human tumor (MOLT 4), a 60 kd protein is detected as well as two proteins of 40 and 45 kd. These two proteins of 40 and 45 kd are also detected in human brain extracts. In BM2 cells, proteins of 50 kd and 20 kd are detected.
Clinical Data
male
Genes Expressed
immunoglobulin; monoclonal antibody; against a synthetic MYB oncogene peptide
Cellular Products
immunoglobulin; monoclonal antibody; against a synthetic MYB oncogene peptide
Comments
Animals were immunized with a synthetic polypeptide with the sequence RHYDEDPEKEKRIKELEL corresponding to peptides 282 to 300 of the MYB oncogene. Spleen cells were fused with Sp2/0 myeloma cells. The antibody reacts with the synthetic MYB oncogene peptide. In immunoblot assays, the antibody recognizes proteins of approximately 80 kd and 60 kd in extracts from MSTF cells (mink lung cells transformed by fes). In human tumor (MOLT 4), a 60 kd protein is detected as well as two proteins of 40 and 45 kd. These two proteins of 40 and 45 kd are also detected in human brain extracts. In BM2 cells, proteins of 50 kd and 20 kd are detected.
Complete Growth Medium
The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Protocol: Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 1 to 2 X 10(5) viable cells/ml.
Interval: Maintain cell density between 1 X 10(5) and 1 X 10(6) viable cells/ml.
Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density)
Cryopreservation
Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO Complete growth medium supplemented with 5% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase
Culture Conditions
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37.0°C
Isotype
IgG1 kappa
Name of Depositor
National Cancer Institute
Deposited As
mouse (B cell); mouse (myeloma)
References

Animals were immunized with the synthetic peptide RHYDEDPEKEKRIKELEL corresponding to residues 282 through 300 of the MYB sequence (formerly residues 94 through 112 of the v-myb sequence).

In immunoblot assays, the antibody recognizes proteins of approximately 80 kd and 60 kd in extracts from MSTF cells (mink lung cells transformed by fes). In human tumor (MOLT 4), a 60 kd protein is detected as well as two proteins of 40 and 45 kd. These two proteins of 40 and 45 kd are also detected in human brain extracts. In BM2 cells, proteins of 50 kd and 20 kd are detected.

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