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133-10F6
133-10F6
規格:
價格:
編號:B163564
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
133-10F6
商品貨號
B163564
Organism
Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Tissue
spleen
培養基
Cell Type
hybridoma: B lymphocyte
Product Format
frozen
Morphology
lymphoblast
Culture Properties
suspension
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Applications
Using immunoblot techniques, the ascites produced from these cells recognized proteins of approximately 45,000 and 75,000 daltons in a variety of transformed mammalian cells.
In addition, proteins of 45,000 and 55,000 were detected in various urine samples.
Animals were immunized with a synthetic polypeptide with the sequence LGEHHCTPSPPVDHG corresponding to peptides 348 to 363 of the c-myb oncogene, formerly v-myb (residues 160 to 175).
Storage Conditions
liquid nitrogen vapor phase
Derivation
Animals were immunized with a synthetic polypeptide with the sequence LGEHHCTPSPPVDHG corresponding to peptides 348 to 363 of the c-myb oncogene, formerly v-myb (residues 160 to 175). Spleen cells were fused with Sp2/0 myeloma cells. Using immunoblot techniques, the ascites produced from these cells recognized proteins of approximately 45,000 and 75,000 daltons in a variety of transformed mammalian cells. In addition, proteins of 45,000 and 55,000 were detected in various urine samples. The IgG1 and IgG2b reactivities of the antibody with the c-myb peptide (348 to 363) were confirmed by ELISA. IgG1 was strongly positive while IgG2b was only slightly reactive. A culture submitted to the ATCC in February of 2001 was found to be contaminated with mycoplasma. Progeny were cured by a 21-day treatment with BM Cycline. The cells were assayed for mycoplasma, by the Hoechst stain, PCR and the standard culture test, after a six-week period following treatment. All tests were negative.
Genes Expressed
immunoglobulin; monoclonal antibody; against a synthetic c-myb oncogene peptide
Cellular Products
immunoglobulin; monoclonal antibody; against a synthetic c-myb oncogene peptide
Tumorigenic
Yes
Effects
Yes, produces ascites in female 129G1X+ x BALB/c mice
Comments
Animals were immunized with a synthetic polypeptide with the sequence LGEHHCTPSPPVDHG corresponding to peptides 348 to 363 of the c-myb oncogene, formerly v-myb (residues 160 to 175). Spleen cells were fused with Sp2/0 myeloma cells. Using immunoblot techniques, the ascites produced from these cells recognized proteins of approximately 45,000 and 75,000 daltons in a variety of transformed mammalian cells. In addition, proteins of 45,000 and 55,000 were detected in various urine samples. The IgG1 and IgG2b reactivities of the antibody with the c-myb peptide (348 to 363) were confirmed by ELISA. IgG1 was strongly positive while IgG2b was only slightly reactive. A culture submitted to the ATCC in February of 2001 was found to be contaminated with mycoplasma. Progeny were cured by a 21-day treatment with BM Cycline. The cells were assayed for mycoplasma, by the Hoechst stain, PCR and the standard culture test, after a six-week period following treatment. All tests were negative.
Complete Growth Medium
The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Protocol: Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 1 to 2 X 10(5) viable cells/ml.
Interval: Maintain cell density between 1 X 10(5) and 1 X 10(6) viable cells/ml.
Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density)
Cryopreservation
Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase
Culture Conditions
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37.0°C
Isotype
IgG1 and 2b kappa
Name of Depositor
National Cancer Institute
Deposited As
mouse (B cell); mouse (myeloma)
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