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14.8
14.8
規格:
價格:
編號:B163567
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
14.8
商品貨號
B163567
Organism
Rattus norvegicus (B cell); Mus musculus (myeloma), rat (B cell); mouse (myeloma)
Cell Type
hybridoma: B lymphocyte
培養基
Product Format
frozen
Morphology
lymphoblast
Culture Properties
suspension
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease
Lymphoma
Applications
This antibody reacts with the CD45R antigen which is preferentially expressed on B cells and their precursors and a subpopulation of T cells.
The antibody binds to the high molecular weight isoform of CD45 expressing all three variable exons.
The antibody is important because it detects B cell precursors in 16 day old fetal mouse liver, and plates coated with this antibody have been used for depletion experiments.
Tested and found negative for ectromelia virus (mousepox).
Derivation
Spleen cells were fused with P3X63Ag8.653 myeloma cells.
Genes Expressed
Tested and found negative for ectromelia virus (mousepox).
immunoglobulin; monoclonal antibody; against mouse B cell (B lymphocyte) precursors (CD45RA),The epitope recognized requires expression of exon A.
Cellular Products
immunoglobulin; monoclonal antibody; against mouse B cell (B lymphocyte) precursors (CD45RA)
Comments
Animals were immunized with W279B murine B lymphoma cells.
Spleen cells were fused with P3X63Ag8.653 myeloma cells.
This antibody reacts with the CD45R antigen which is preferentially expressed on B cells and their precursors and a subpopulation of T cells.
The epitope recognized requires expression of exon A.
The antibody binds to the high molecular weight isoform of CD45 expressing all three variable exons.
Thy-1 positive peripheral T cells are positive with this antibody.
The antibody is important because it detects B cell precursors in 16 day old fetal mouse liver, and plates coated with this antibody have been used for depletion experiments.
Tested and found negative for ectromelia virus (mousepox).
Complete Growth Medium
The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 1 x 10 exp5 cells/ml ay maintain between 1 x 10 exp5 and 1 x 10 exp6 cells/ml.
Isotype
IgG2b
Name of Depositor
PW Kincade
Deposited As
rat (B cell); mouse (myeloma)
References

Kincade PW, et al. Antigens displayed on murine B lymphocyte precursors. J. Immunol. 127: 2262-2268, 1981. PubMed: 6975314

Landreth KS, et al. Enrichment of human marrow lymphocytes with monoclonal antibodies to murine antigens. Proc. Natl. Acad. Sci. USA 79: 2370-2374, 1982. PubMed: 7048318

Luqman M, et al. Differential expression of the alternatively spliced exons of murine CD45 in Th1 and Th2 cell clones. Eur. J. Immunol. 21: 17-22, 1991. PubMed: 1671357

Kincade PW. Formation of B lymphocytes in fetal and adult life. Adv. Immunol. 31: 177-245, 1981. PubMed: 7032254

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