Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Applications
Tested and found negative for ectromelia virus (mousepox).
Derivation
Animals were immunized with purified human IgG1 (kappa light chain) myeloma protein.
Spleen cells were fused with Sp2/0-Ag14 myeloma cells.
Genes Expressed
immunoglobulin; monoclonal antibody; against human kappa light chain
Cellular Products
immunoglobulin; monoclonal antibody; against human kappa light chain
Comments
Animals were immunized with purified human IgG1 (kappa light chain) myeloma protein.
Spleen cells were fused with Sp2/0-Ag14 myeloma cells.
Tested and found negative for ectromelia virus (mousepox).
Complete Growth Medium
The base medium for this cell line is ATCC Hybri-Care Medium, Catalog No. 46-X. Hybri-Care Medium is supplied as a powder and should be reconstituted in 1 L cell culture grade water. To make the complete growth medium, add the following components to the base medium:
fetal bovine serum to a final concentration of 10%
1.5 g/L sodium bicarbonate for use with 5% CO2 in air atmosphere.
Subculturing
Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
