最新中文字幕-精品国产99-国产精品福利在线观看-粉嫩av一区二区三区-日韩精品久久久久-美丽的姑娘国语视频-亚洲欧洲色-国产不卡毛片-三级黄毛片-免费福利在线-日韩av三区-欧美成年人网站-欧洲美女与动交ccoo-成人涩涩软件-秋霞影院午夜老牛影院-婷婷久久伊人-呦呦视频在线观看-www.天天综合-日韩精品高清视频-国产欧美综合在线-欧美日韩一区二区三区在线视频-a猛片免费播放-91九色蝌蚪国产-国产欧美日韩专区-嫩模被强到高潮呻吟不断

歷史搜索:A549    293T 金黃色葡萄球菌 大腸桿菌 AKK菌
購(gòu)物車 1 種商品 - 共0元
當(dāng)前位置: 首頁(yè) > ATCC代理 > 142-24E5 [1/24E05, 142-24E05, SCRF 35.1] B163570
142-24E5 [1/24E05, 142-24E05, SCRF 35.1]
142-24E5 [1/24E05, 142-24E05, SCRF 35.1]
規(guī)格:
價(jià)格:
編號(hào):B163570
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱
142-24E5 [1/24E05, 142-24E05, SCRF 35.1]
商品貨號(hào)
B163570
Organism
Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Cell Type
hybridoma: B lymphocyte
培養(yǎng)基
Product Format
frozen
Morphology
lymphoblast
Culture Properties
suspension but many cells attach
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Applications
Animals were immunized with a synthetic polypeptide with the sequence YREQIKRVKDSDDVPMVLVGNKC corresponding to peptides 96 to 118 of the predicted p21 sequence of the ras (Ha-ras) oncogene, formerly v-ras (H).
The antibody reacts in indirect fluorescence assays of frozen sections and peroxidase stain paraffin embedded cell lines or tissues.
Images
Derivation
Spleen cells were fused with Sp2/0 myeloma cells.
Genes Expressed
immunoglobulin; monoclonal antibody; against a synthetic H/N-ras oncogene peptide
Cellular Products
immunoglobulin; monoclonal antibody; against a synthetic H/N-ras oncogene peptide
Comments
Animals were immunized with a synthetic polypeptide with the sequence YREQIKRVKDSDDVPMVLVGNKC corresponding to peptides 96 to 118 of the predicted p21 sequence of the ras (Ha-ras) oncogene, formerly v-ras (H).
Spleen cells were fused with Sp2/0 myeloma cells.
The antibody recognizes a 23,000 dalton protein in all mammalian cell lines tested, a 38,000 dalton in yeast cells, and proteins of 100,000, 55,000 and 23,000 daltons in the urine of a variety of individuals.
The antibody reacts in indirect fluorescence assays of frozen sections and peroxidase stain paraffin embedded cell lines or tissues.
A culture submitted to the ATCC in February of 2001 was found to be contaminated with mycoplasma. Progeny were cured by a 21-day treatment with BM Cycline.
The cells were assayed for mycoplasma, by the Hoechst stain, PCR and the standard culture test, after a six-week period following treatment. All tests were negative.
Complete Growth Medium
The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density)
Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 1 to 2 X 10 exp5 viable cells/ml.
Maintain cell density between 5 X 10 exp4 and 1 to 2 X 10 exp6 viable cells/ml.
Cryopreservation
Culture medium with 45% fetal bovine serum, 90%; DMSO, 10%
Culture Conditions
Temperature: 37.0°C
Isotype
IgG1; kappa light chain
Name of Depositor
National Cancer Institute
Deposited As
mouse (B cell); mouse (myeloma)
References

Niman HL, Lerner RA. Polypeptide-induced monoclonal receptors to protein ligands. US Patent 5,015,571 dated May 14 1991

Niman HL, Lerner RA. Polypeptide-induced monoclonal receptors to protein ligands. US Patent 5,030,565 dated Jul 9 1991

Chesa PG, et al. Expression of p21ras in normal and malignant human tissues: lack of association with proliferation and malignancy. Proc. Natl. Acad. Sci. USA 84: 3234-3248, 1984. PubMed: 3554234

Habib NA, Wood CB. The expression of ras oncogene in normal and pathological liver disease. Int. Surg. 71: 182-183, 1986. PubMed: 3771121

Bokoch GM, Parkos CA. Identification of novel GTP-binding proteins in the human neutrophil. FEBS Lett. 227: 66-70, 1988. PubMed: 3123273

Luo JC, et al. Expression p21 ras-related protein in the plasma and tissue of patients with adenomas and carcinomas of the colon. Biomarkers 1: 29-33, 1996.

  • 王經(jīng)理
    手機(jī)號(hào)：17280875617
    QQ：1438578920
  • 胡經(jīng)理
    手機(jī)號(hào)：13345964880
    QQ：2438244627
  • 周經(jīng)理
    手機(jī)號(hào)：17757487661
    QQ：1296385441
  • 于經(jīng)理
    手機(jī)號(hào)：18067160830
    QQ：2088210172
  • 沈經(jīng)理
    手機(jī)號(hào)：19548299266
    QQ：2662369050
  • 李經(jīng)理
    手機(jī)號(hào)：13626845108
    QQ：972239479