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143-4-2
143-4-2
規(guī)格:
價(jià)格:
編號(hào):B163572
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱
143-4-2
商品貨號(hào)
B163572
Organism
Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Cell Type
hybridoma: B lymphocyte
培養(yǎng)基
Product Format
frozen
Morphology
lymphoblast
Culture Properties
suspension
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Applications
The line secretes an allele specific antibody that reacts with the Ly 6.2C mouse T cell surface antigen.
The antibody is useful for studies of T cell activation and T cell surface antigen phenotypes.
Derivation
Spleen cells were fused with Sp2/0-Ag14 myeloma cells.
Genes Expressed
immunoglobulin; monoclonal antibody; against mouse Ly 6.2C
Cellular Products
immunoglobulin; monoclonal antibody; against mouse Ly 6.2C
Comments
Animals were immunized with the BM10-37 mouse cytotoxic T lymphocyte (CTL) cell clone (anti H-2 Kb).
Spleen cells were fused with Sp2/0-Ag14 myeloma cells.
The line secretes an allele specific antibody that reacts with the Ly 6.2C mouse T cell surface antigen.
The antibody promotes alloreactive CTL lysis of antigen non-bearing targets.
It does not appear to interact directly with the T cell antigen receptor (TcR) as Ly 6.2C is not believed to be associated with the TcR complex on the T cell surface.
The antibody is useful for studies of T cell activation and T cell surface antigen phenotypes.
Complete Growth Medium
Iscove's modified Dulbecco's medium, 90%; fetal bovine serum, 10%
Subculturing
Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
Isotype
IgG1
Name of Depositor
JA Bluestone
Deposited As
mouse (B cell); mouse (myeloma)
References

Leo O, et al. Activation of murine T lymphocytes with monoclonal antibodies: detection on Lyt-2+ cells of an antigen not associated with the T cell receptor complex but involved in T cell activation. J. Immunol. 139: 1214-1222, 1987. PubMed: 3497199

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