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Animals were immunized with a synthetic peptide with the sequence YTLVREIRQYRLKKESKEEKTPGC corresponding to peptides 157-180 of the v-ras K oncogene.
Spleen cells were fused with Sp2/0 myeloma cells.
The antibody reacts with the synthetic v-ras K oncogene peptide. In ELISA testing, the antibody weakly detects the homologous region in the ras H sequence. In immunoblot testing a serum protein of approximately 45 kd is detected.
The IgG1 and IgG2b reactivities of the antibody with the v-ras K peptide (157-180) was confirmed by ELISA. IgG1 was strongly positive while IgG2b was only slightly positive.
