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182-PF SK
182-PF SK
規格:
價格:
編號:B163605
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
182-PF SK
商品貨號
B163605
Organism
Homo sapiens, human
Tissue
skin
培養基
Product Format
frozen
Morphology
fibroblast
Culture Properties
adherent
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease
hereditary adenomatosis
Age
20 years
Gender
male
Ethnicity
Caucasian
Clinical Data
The tissue donor suffered from hereditary adenomatosis.
male
Caucasian
20 years
Comments
The tissue donor suffered from hereditary adenomatosis.
The line is capable of 10 population doublings after thawing.
Complete Growth Medium
The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:3 is recommended
Medium Renewal: Twice per week
Remove medium, add fresh 0.25% trypsin solution for 1 to 3 minutes, remove trypsin and let the culture sit at room temperature for 5 to 10 minutes. Add fresh medium, aspirate and dispense into new flasks. Subculture every 10 days.
Name of Depositor
L Kopelovich
Deposited As
Homo sapiens
References

Rhim JS, et al. Chemical transformation of cultured skin fibroblasts from humans genetically predisposed to cancer. Cancer Detect. Prev. 4: 239-247, 1981. PubMed: 7349781

Prog. Cancer Res. Ther. 13: 97-108, 1980.

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