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1A2
1A2
規格:
價格:
編號:B163612
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
1A2
商品貨號
B163612
Organism
Homo sapiens, human
Cell Type
lymphoblast B lymphocyte; Epstein-Barr virus (EBV) transforme
培養基
Product Format
frozen
Morphology
lymphoblast
Culture Properties
suspension
Biosafety Level
2 CELLS CONTAIN HERPESVIRUS

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease
Lymphoma
Applications
This line was derived from the GM1500 lymphoma line.
The cells are resistant to 6-thioguanine.
The cells can be used as a source of infectious EBV, and can be used as driving the cell in"cell driven" EBV transformation of human B cells.
Storage Conditions
liquid nitrogen vapor phase
Disclosure
This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC.
Derivation
This line was derived from the GM1500 lymphoma line.
Genes Expressed
Epstein-Barr virus (EBV)
Cellular Products
Epstein-Barr virus (EBV)
Comments
This line was derived from the GM1500 lymphoma line.
The cells are resistant to 6-thioguanine.
The cells can be used as a source of infectious EBV, and can be used as driving the cell in"cell driven" EBV transformation of human B cells.
Complete Growth Medium
RPMI 1640 medium with 1 mM sodium pyruvate and 2 mM L-glutamine, 85%; fetal bovine serum, 15%
Subculturing
Protocol: Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10(5) cells/ml and maintain between 2 X 10(5) and 1 X 10(6) cells/ml.
Interval: every 3 days
Medium Renewal: Every 3 days
Cryopreservation
Freeze medium: culture medium, 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor phase
Culture Conditions
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37.0°C
Population Doubling Time
24 hrs (when cells are in logarithmic growth)
Name of Depositor
Genetic Systems Corp.
Deposited As
Homo sapiens
U.S. Patent Number
References

Lostrom ME. Cell-driven viral transfer in eukaryotes. US Patent 4,464,465 dated Aug 7 1984

Croce CM, et al. Chromosomal location of the genes for human immunoglobulin heavy chains. Proc. Natl. Acad. Sci. USA 76: 3416-3419, 1979. PubMed: 114999

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