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1B2-1B7
1B2-1B7
規格:
價格:
編號:B163614
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
1B2-1B7
商品貨號
B163614
Organism
Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Cell Type
hybridoma: B lymphocyte
培養基
Product Format
frozen
Morphology
lymphoblast
Culture Properties
suspension
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Applications
The antibody is specific for the non-reducing terminal N-acetyllactosamine and does not react with glycolipids having a type I chain, a ganglio series structure or a sialosyl or fucosyl substitution at the N-acetyllactosamine residue.
Tested and found negative for ectromelia virus (mousepox).
Derivation
Spleen cells were fused with NS-1 myeloma cells.
Genes Expressed
immunoglobulin; monoclonal antibody; against glycosphingolipid
Cellular Products
immunoglobulin; monoclonal antibody; against glycosphingolipid
Comments
Animals were immunized with lacto-N-nor-hexaosylceramide non-covalently adsorbed to naked Salmonella minnesota.
Spleen cells were fused with NS-1 myeloma cells.
The antibody is specific for the non-reducing terminal N-acetyllactosamine and does not react with glycolipids having a type I chain, a ganglio series structure or a sialosyl or fucosyl substitution at the N-acetyllactosamine residue.
The antibody agglutinates all adult human erythrocytes but not umbilical cord blood erythrocytes.
Tested and found negative for ectromelia virus (mousepox).
Complete Growth Medium
RPMI 1640 medium, 85%; fetal bovine serum, 15%
Subculturing
Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 1 x 10 exp5 cells/ml and maintain between 1 x 10 exp5 and 1 x 10 exp6 cells/ml.
Isotype
IgM
Name of Depositor
WW Young
Deposited As
mouse (B cell); mouse (myeloma)
References

Young WW Jr., et al. Two monoclonal anticarbohydrate antibodies directed to glycosphingolipids with a lacto-n-glycosyl type II chain. J. Biol. Chem. 256: 10967-10972, 1981. PubMed: 7287743

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