Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Applications
The antibody reacts with TNP and forms rosettes with TNP conjugated sheep red blood cells.
Tested and found negative for ectromelia virus (mousepox).
Storage Conditions
liquid nitrogen vapor phase
Derivation
Spleen cells were fused with P3X63Ag8.653 myeloma cells.
Genes Expressed
immunoglobulin; monoclonal antibody; against 2,4,6-trinitrophenyl (TNP)
Cellular Products
immunoglobulin; monoclonal antibody; against 2,4,6-trinitrophenyl (TNP)
Comments
Animals were immunized with TNP-KLH.
Spleen cells were fused with P3X63Ag8.653 myeloma cells.
The antibody reacts with TNP and forms rosettes with TNP conjugated sheep red blood cells.
Tested and found negative for ectromelia virus (mousepox).
Complete Growth Medium
The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 1 x 10 exp5 cells/ml and maintain between 1 x 10 exp5 and 1 x 10 exp6 cells/ml.
