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Raghavan M, et al. Investigation of the interaction between the class I MHC-related Fc receptor and its immunoglobulin G ligand. Immunity 1: 303-315, 1994. PubMed: 7889418The cell line was established by Louis Gastinel and Pamela Bjorkman in 1992. Animals were immunized with and twice boosted with soluble neonatal Fc receptor (FcRn) in an adjuvant. Three days before fusion one mouse was boosted with purified FcRn. Spleen cells were fused with HL-1 Friendly myeloma cells (a derivative of P3X63Ag8.653 that is deficient in both hypoxanthine phosphoribosyl transferase [HPRT] and adenine phosphoribosyl transferase [APRT]).
FcRn is a heterodimer composed of a membrane bound heavy chain attached noncovalently to beta 2-microgloublin.
The antibody recognizes FcRn heavy chain heterodimers.
FcRn is a heterodimer composed of a membrane bound heavy chain attached noncovalently to beta 2-microgloublin. It is structurally similar to class I major histocompatibility (MHC) molecules.
The IG3 (ATCC CRL-2434) antibody recognizes two lipid linked forms of FcRn.
These forms are truncated FcRn heavy chain paired with beta 2-microgloublin-DAF (FcRn beta-2 microglobulin DAF) and lipid-linked FcRn paired with unaltered beta 2-microgloublin (FcRn-DAF/beta 2-microgloublin).
It also recognizes the FcRn-DAF construct.
The 2G3 (ATCC CRL-2435) antibody fails to recognize the FcRn heterodimer form in which the heavy chain is attached to the lipid anchor (FcRn-DAF/beta 2-microgloublin) and it also fails to recognize the FcRn-DAF construct.
Unlike 4C9 (ATCC CRL-2437), the monoclonal antibodies IG3 (ATCC CRL-2434) and 2G3 (ATCC CRL-2435) do not recognize rat beta 2-microglobulin alone or complexed with FcRn heavy chains.
Subcultures are prepared by scraping. Dislodge cells from the flask substrate with a cell scraper. Aspirate gently and dispense into new flasks. Seed flasks at 2 x 105 viable cells/mL.
Subcultivation Ratio: A subcultivation ratio of 1:5 every 3 to 4 day is recommended
Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density).
Raghavan M, et al. Investigation of the interaction between the class I MHC-related Fc receptor and its immunoglobulin G ligand. Immunity 1: 303-315, 1994. PubMed: 7889418
