Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Applications
The antibody reacts with a cell surface molecule (CD62L) that mediates the initial adhesion of lymphocytes and neutrophils to endothelial cells.
The antibody can be used in both immunofluorescence and immunoprecipitation assys.
The cells can be adapted to serum free growth in HB101 medium.
Derivation
Spleen cells were fused with Sp2/0-Ag14 myeloma cells.
Genes Expressed
immunoglobulin; monoclonal antibody; against human CD62L
Cellular Products
immunoglobulin; monoclonal antibody; against human CD62L
Comments
Animals were immunized with human peripheral blood leukocytes.
Spleen cells were fused with Sp2/0-Ag14 myeloma cells.
The antibody reacts with a cell surface molecule (CD62L) that mediates the initial adhesion of lymphocytes and neutrophils to endothelial cells.
The antibody can be used in both immunofluorescence and immunoprecipitation assys.
The cells can be adapted to serum free growth in HB101 medium.
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
