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2.4G2
2.4G2
規(guī)格:
價格:
編號:B163631
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱
2.4G2
商品貨號
B163631
Organism
Rattus norvegicus (B cell); Mus musculus (myeloma), rat (B cell); mouse (myeloma)
Cell Type
Hybridoma: B lymphocyte
培養(yǎng)基
Product Format
frozen
Morphology
Lymphoblast-like
Culture Properties
Suspension
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Applications
The antibody can be used to block non-specific binding to Fc gamma bearing cells.
Storage Conditions
liquid nitrogen vapor phase
Derivation

Animals were immunized with the J774 mouse macrophage cell line. 

Spleen cells were fused with P3U1 myeloma cells.

Genes Expressed
immunoglobulin; monoclonal antibody; against the Fc gamma receptor (FcRII, CD32)
Cellular Products
immunoglobulin; monoclonal antibody; against the Fc gamma receptor (FcRII, CD32)
Tumorigenic
Yes
Effects
Yes, in irradiated pristane primed BALB/c mice.
Comments

The antibody reacts with and immunoprecipitates the 50000 dalton to 70000 dalton Fc gamma receptor on macrophages and Fc gamma bearing lymphoid cells.

Tested and found negative for ectromelia virus (mousepox).

Complete Growth Medium
Dulbecco's modified Eagle's medium with 4 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate and 4.5 g/L glucose, 90%; horse serum, 5%; fetal bovine serum, 5%
Subculturing
Cultures can be maintained by addition of fresh medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 1 x 10viable cells/mL. Maintain cultures at a cell concentration between 1 x 105 and 1 x 10cells/mL. Do not allow the cell concentration to exceed 1 x 10cells/mL.
Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density)
Cryopreservation
culture medium 95%; DMSO, 5%
Culture Conditions
Temperature: 37°C
Atmosphere: Air, 95%; Carbon dioxide (CO2), 5%
Isotype
IgG2b
Name of Depositor
JC Unkeless
Deposited As
rat (B cell); mouse (myeloma)
References

Unkeless JC. Characterization of a monoclonal antibody directed against mouse macrophage and lymphocyte Fc receptors. J. Exp. Med. 150: 580-596, 1979. PubMed: 90108

Mellman IS, Unkeless JC. Purification of a functional mouse Fc receptor through the use of a monoclonal antibody. J. Exp. Med. 152: 1048-1069, 1980. PubMed: 6158545

Nussenzweig MC, et al. Studies of the cell surface of mouse dendritic cells and other leukocytes. J. Exp. Med. 154: 168-187, 1981. PubMed: 7252426

Yoshikai Y, et al. Clonal expansion of superantigen-reactive T cells is resistant to FK506 in mice with AIDS. J. Virol. 71: 746-749, 1997. PubMed: 8985410

Wilson ME, et al. Local suppression of IFN-gamma in hepatic granulomas correlates with tissue-specific replication of Leishmania chagasi. J. Immunol. 156: 2231-2239, 1996. PubMed: 8690913

Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC.

Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988.

Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC.

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