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20.3 [TAb 250]
20.3 [TAb 250]
規格:
價格:
編號:B163632
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
20.3 [TAb 250]
商品貨號
B163632
Organism
Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Cell Type
hybridoma:lymphocyte
培養基
Product Format
frozen
Morphology
lymphoblast
Culture Properties
suspension
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Applications
The hybridoma cell line named 20.3 produces monoclonal antibody TAb 250.
The TAb 250 monoclonal antibody is specific for an extracellular epitope of the c-erbB-2 protein (gp185).
Derivation
Spleen cells were fused with P3X63Ag8.653 mouse myeloma cells.
Genes Expressed
immunoglobulin; monoclonal antibody; against human c-erb B2 protein
Cellular Products
immunoglobulin; monoclonal antibody; against human c-erb B2 protein
Tumorigenic
Yes
Effects
Yes, in pristane primed BALB/c mice
Comments
Animals were immunized with NIH3T3 cells transfected with the human c-erb B2 oncogene.
Spleen cells were fused with P3X63Ag8.653 mouse myeloma cells.
The hybridoma cell line named 20.3 produces monoclonal antibody TAb 250.
The TAb 250 monoclonal antibody is specific for an extracellular epitope of the c-erbB-2 protein (gp185). It inhibits in a dose-dependent manner the in vitro proliferation of human breast tumor cell lines that overexpress c-erbB-2.
A culture deposited with the ATCC as HB-10646 in January of 1991 was found to be contaminated with mycoplasma. Progeny were cured by a 21-day treatment with BM Cycline.
The cured cell line is available as CRL-2655. The original patent deposit is available as HB-10646.
Complete Growth Medium
The base medium for this cell line is ATCC-formulated Iscove's Modified Dulbecco's Medium, Catalog No. 30-2005. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 20%.
Subculturing
Medium Renewal: Every 2 to 3 days
Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 2 X 10 exp5 viable cells/ml.
Maintain cell density between 1 X 10 exp5 and 1 X 10 exp6 viable cells/ml.
Cryopreservation
culture medium 95%; DMSO, 5%
Culture Conditions
Temperature: 37.0°C
Isotype
IgG1; kappa light chain
Name of Depositor
Berlex Laboratories, Inc.
Deposited As
mouse (B cell); mouse (myeloma)
References

Shawver LK, et al. Anti-neoplastic drugs in cancer therapy. US Patent 6,123,939 dated Sep 26 2000

Hancock MC, et al. A monoclonal antibody against the c-erbB-2 protein enhances the cytotoxicity. Cancer Res. 51: 4575-4580, 1991. PubMed: 1678683

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