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200-3-G6-4 (20.4)
200-3-G6-4 (20.4)
規格:
價格:
編號:B163633
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
200-3-G6-4 (20.4)
商品貨號
B163633
Organism
Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Cell Type
hybridoma: B lymphocyte
培養基
Product Format
frozen
Morphology
lymphoblast
Culture Properties
suspension
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Applications
The antibody binds to the 75000 dalton NGF receptor of primates.
Storage Conditions
liquid nitrogen vapor phase
Disclosure
This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC.
Derivation
Spleen cells were fused with P3X63Ag8 myeloma cells.
Genes Expressed
immunoglobulin; monoclonal antibody; against nerve growth factor (NGF) receptor of primates
Cellular Products
immunoglobulin; monoclonal antibody; against nerve growth factor (NGF) receptor of primates
Tumorigenic
Yes
Effects
Yes, forms ascites in BALB/c mice
Comments
Animals were immunized with human melanoma cell lines.
Spleen cells were fused with P3X63Ag8 myeloma cells.
The antibody binds to the 75000 dalton NGF receptor of primates.
Complete Growth Medium
Iscove's modified Dulbecco's medium with 2 mM L-glutamine, 90%; fetal bovine serum, 10%
Subculturing
Protocol: Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 1 X 10(5) cells/ml and maintain between 1 X 10(5) and 1 X 10(6) cells/ml.
Medium Renewal: every 2 to 3 days
Cryopreservation
Freeze medium: Culture medium, 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor phase
Culture Conditions
Temperature: 37.0°C
Isotype
IgG1
Name of Depositor
Wistar Institute
Deposited As
mouse (B cell); mouse (myeloma)
U.S. Patent Number
References

Koprowski H, et al. Diagnostic method for detection of neural crest disease. US Patent 4,786,593 dated Nov 22 1988

Johnson EM Jr.. Tumor diagnostic method. US Patent 4,855,241 dated Aug 8 1989

Levi AD, et al. The functional characteristics of Schwann cells cultured from human peripheral nerve after transplantation into a gap within the rat sciatic nerve. J. Neurosci. 14: 1309-1319, 1994. PubMed: 8120626

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