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2254-62.2
2254-62.2
規格:
價格:
編號:B163643
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
2254-62.2
商品貨號
B163643
Organism
hamster
Tissue
kidney
培養基
Product Format
frozen
Morphology
fibroblast
Culture Properties
adherent
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Applications
This line was derived from BHK-21(c-13) (see ATCC CCL-10).
This line has been used as a host for transformation with expression vectors containing selectable and amplifiable marker DNAs (e.
Disclosure
This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC.
Derivation
This line was derived from BHK-21(c-13) (see ATCC CCL-10).
Comments
This line was derived from BHK-21(c-13) (see ATCC CCL-10).
This line has been used as a host for transformation with expression vectors containing selectable and amplifiable marker DNAs (e.g., Factor VIII).
Complete Growth Medium
Dulbecco's modified Eagle's medium with 4 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate and 4.5 g/L glucose and supplemented with 100 nM methotrexate, 93%; dialyzed fetal bovine serum, 7%
Subculturing
Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:8 is recommended
Medium Renewal: Every 2 to 3 days
Remove medium, rinse with fresh 0.25% trypsin, remove trypsin and incubate the flask at 37C until the cells detach (about 5 to 10 minutes). Add fresh medium, aspirate and dispense into new flasks.
Culture Conditions
Temperature: 37.0°C
Name of Depositor
Genentech, Inc.
Deposited As
hamster
U.S. Patent Number
References

Capon DJ, et al. Preparation of functional human factor VIII in mammalian cells using methotrexate based selection. US Patent 4,965,199 dated Oct 23 1990

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