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24IA2E2E10D5 [24IA2]
24IA2E2E10D5 [24IA2]
規(guī)格:
價(jià)格:
編號:B163648
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱
24IA2E2E10D5 [24IA2]
商品貨號
B163648
Organism
Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Cell Type
hybridoma: B lymphocyte
培養(yǎng)基
Product Format
frozen
Morphology
lymphoblast
Culture Properties
suspension
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease
Leukemia
Applications
The antibody reacts with the A, B, C, AB and ABC strains of FeLV.
Disclosure
This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC.
Derivation
Animals were immunized with purified FeLV p27.
Spleen cells were fused with Sp2/0-Ag14 myeloma cells.
Genes Expressed
immunoglobulin; monoclonal antibody; against the p27 protein of Feline Leukemia virus (FeLV)
Cellular Products
immunoglobulin; monoclonal antibody; against the p27 protein of Feline Leukemia virus (FeLV)
Tumorigenic
Yes
Effects
Yes, forms ascites in pristane primed BALB/c mice
Comments
Animals were immunized with purified FeLV p27.
Spleen cells were fused with Sp2/0-Ag14 myeloma cells.
The antibody reacts with the A, B, C, AB and ABC strains of FeLV.
The antibody does not react with RD114 virus or AKR Murine Leukemia virus (MuLV).
Complete Growth Medium
The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
Isotype
IgG
Name of Depositor
Regents of the University of California
Deposited As
mouse (B cell); mouse (myeloma)
U.S. Patent Number
References

Lutz H. Hybridomas producing monoclonal antibodies specific for FeLV p27. US Patent 4,713,325 dated Dec 15 1987

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