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2A3A1H

規格:

價格:

編號:B163669

品牌:Mingzhoubio

標準菌株

定量菌液

DNA

RNA

規格:

凍干粉

斜面

甘油

平板

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產品名稱

2A3A1H

商品貨號

B163669

Organism

Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)

Cell Type

hybridoma: B lymphocyte

培養基

Product Format

frozen

Morphology

lymphoblast

Culture Properties

suspension

Biosafety Level

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Strain

Strain:? BALB/c (B cell); BALB/c (myeloma)

Applications

The antibody binds to IL-2 receptors on human leukocytes and blocks both mitogen induced and antigen induced PBL proliferation.

Disclosure

This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC.

Derivation

Spleen cells were fused with NS-1 myeloma cells.

Genes Expressed

immunoglobulin; monoclonal antibody; against human interleukin 2 (interleukin-2, IL-2) receptor

Cellular Products

immunoglobulin; monoclonal antibody; against human interleukin 2 (interleukin-2, IL-2) receptor

Comments

Animals were immunized with phytohemagglutinin activated human peripheral blood (PBL) leukocytes.

Spleen cells were fused with NS-1 myeloma cells.

The antibody binds to IL-2 receptors on human leukocytes and blocks both mitogen induced and antigen induced PBL proliferation.

Complete Growth Medium

RPMI 1640 medium with 2 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate, 4.5 g/L glucose, 10 mM HEPES, and 1.0 mM sodium pyruvate and supplemented with 0.025 mM 2-mercaptoethanol, 90%; fetal bovine serum, 10%

Subculturing

Medium Renewal: Every 2 to 3 days

Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 6 X 10 exp5 cells/ml.

Cryopreservation

Culture medium, 95%; DMSO, 5%

Culture Conditions

Temperature: 37.0°C

Isotype

IgG1

Name of Depositor

Immunex Corporation

Deposited As

mouse (B cell); mouse (myeloma)

U.S. Patent Number

4,578,335

References

March CJ, et al. Hybridoma antibody which binds IL-2 receptor. US Patent 4,845,198 dated Jul 4 1989

March CJ, et al. Interleukin-2 receptor. US Patent 4,578,335 dated Mar 25 1986

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