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2D7F10
2D7F10
規格:
價格:
編號:B163680
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
2D7F10
商品貨號
B163680
Organism
Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Cell Type
hybridoma: B lymphocyte
培養基
Product Format
frozen
Morphology
lymphoblast
Culture Properties
suspension
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Applications
The antibody binds to agglutinates L. interogans serovar pomona type kennewicki, and binds to type kennewicki antigen in ELISA.
It can be used to identify type kennewicki in veterinary biologicals.
Derivation
Spleen cells were fused with Sp2/0-Ag14 myeloma cells.
Genes Expressed
immunoglobulin; monoclonal antibody; against Leptospira pomona type kennewicki
Cellular Products
immunoglobulin; monoclonal antibody; against Leptospira pomona type kennewicki
Tumorigenic
Yes
Effects
Yes, forms ascites in BALB/c mice
Comments
Animals were immunized with.
Spleen cells were fused with Sp2/0-Ag14 myeloma cells.
The antibody binds to agglutinates L. interogans serovar pomona type kennewicki, and binds to type kennewicki antigen in ELISA.
It can be used to identify type kennewicki in veterinary biologicals.
Complete Growth Medium
Dulbecco's modified Eagle's medium with 4.5 g/L glucose, 92.5%; horse serum, 5%; fetal bovine serum, 2.5%
Subculturing
Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
Isotype
IgA
Name of Depositor
KW Ruby
Deposited As
mouse (B cell); mouse (myeloma)
References

. . Proceedings of the 94th Annual Meeting of the United States Animal Health Association, Denver, CO, : 290-294, 1990.

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