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2Hx-2
2Hx-2
規格:
價格:
編號:B163690
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
2Hx-2
商品貨號
B163690
Organism
Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Cell Type
hybridoma: B lymphocyte
培養基
Product Format
frozen
Morphology
lymphoblast
Culture Properties
suspension
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Strain

Strain:? BALB/c (B cell); BALB/c (myeloma)

Applications
The antibody reacts with adenoviruses of human subgroups A through E as well as with simian, bovine, canine and swine adenoviruses.
Disclosure
This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC.
Derivation
Spleen cells were fused with Sp2/0-Ag14 myeloma cells.
Genes Expressed
immunoglobulin; monoclonal antibody; against adenovirus group specific antigen
Cellular Products
immunoglobulin; monoclonal antibody; against adenovirus group specific antigen
Tumorigenic
Yes
Effects
Yes, forms ascites in BALB/c mice
Comments
Animals were immunized with Hela cells that were infected with adenovirus 2.
Spleen cells were fused with Sp2/0-Ag14 myeloma cells.
The antibody reacts with adenoviruses of human subgroups A through E as well as with simian, bovine, canine and swine adenoviruses.
It does not react with avian adenoviruses.
Complete Growth Medium
The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
Cryopreservation
culture medium 95%; DMSO, 5%
Culture Conditions
Temperature: 37.0°C
Isotype
IgG2a
Name of Depositor
Massachusetts Institute of Technology
U.S. Patent Number
References

Cepko CL, et al. Immunoprecipitation with two-dimensional pools as a hybridoma screening technique: Production and characterization of monoclonal antibodies against adenovirus 2 proteins. Virology 110: 385-401, 1981. PubMed: 7222481

Cepko CL, et al. Production and use of monoclonal antibodies against adenoviruses. US Patent 4,487,829 dated Dec 11 1984

Cepko CL, et al. Adenovirus hexon monoclonal antibody that is group specific and potentially useful as a diagnostic reagent. J. Clin. Microbiol. 17: 360-364, 1983. PubMed: 6339552

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