最新中文字幕-精品国产99-国产精品福利在线观看-粉嫩av一区二区三区-日韩精品久久久久-美丽的姑娘国语视频-亚洲欧洲色-国产不卡毛片-三级黄毛片-免费福利在线-日韩av三区-欧美成年人网站-欧洲美女与动交ccoo-成人涩涩软件-秋霞影院午夜老牛影院-婷婷久久伊人-呦呦视频在线观看-www.天天综合-日韩精品高清视频-国产欧美综合在线-欧美日韩一区二区三区在线视频-a猛片免费播放-91九色蝌蚪国产-国产欧美日韩专区-嫩模被强到高潮呻吟不断

歷史搜索:A549    293T 金黃色葡萄球菌 大腸桿菌 AKK菌
購物車 1 種商品 - 共0元
當前位置: 首頁 > ATCC代理 > 31-3-4S B163699
31-3-4S
31-3-4S
規(guī)格:
價格:
編號:B163699
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱
31-3-4S
商品貨號
B163699
Organism
Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Cell Type
hybridoma: B lymphocyte
培養(yǎng)基
Product Format
frozen
Morphology
lymphoblast
Culture Properties
suspension
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Applications
The antibody reactivity corresponds to the H-2.31 private serologic specificity.
Tested and found negative for ectromelia virus (mousepox).
Derivation
Spleen cells were fused with Sp2/0-Ag14 myeloma cells.
Genes Expressed
immunoglobulin; monoclonal antibody; against H-2 K d
Cellular Products
immunoglobulin; monoclonal antibody; against H-2 K d
Comments
Animals were immunized with spleen cells from BALB/c mice.
Spleen cells were fused with Sp2/0-Ag14 myeloma cells.
The antibody reactivity corresponds to the H-2.31 private serologic specificity.
The antibody is cytotoxic.
Tested and found negative for ectromelia virus (mousepox).
Complete Growth Medium
Culture Medium: Modified Dulbecco's medium (see below), 90%; fetal bovine
serum, 10%.
DME w HEPES (10mM) 100 mL
L-Glutamine (100x) 1 mL
Solution I 1 mL
Non-essential amino acids (100x) 1 mL
NCTC 135 10 mL
Fetal Bovine Serum 12 mL
Solution I (100x)
1. 1320 mg oxalacetic acid (100 mM, MW 132).
2. 80 mg crystalline bovine insulin (20 units/mL, 25 units/mg).
Add 1 and 2, stir at 37°C. Add Na pyruvate 550 mg (50 mM, FW 110). Bring up to
100 mL with distilled water. Stir at 37°C until solution dissolves. Filter,
aliquot and store frozen.
Subculturing
Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
Isotype
IgM
Name of Depositor
DH Sachs, K Ozato
Deposited As
mouse (B cell); mouse (myeloma)
References

Ozato K, et al. Monoclonal antibodies to mouse MHC antigens. IV. A series of hybridoma clones producing anti-H-2d antibodies and an examination of expression of H-2d antigens on the surface of these cells. Transplantation 34: 113-118, 1982. PubMed: 7135466

  • 王經(jīng)理
    手機號：17280875617
    QQ：1438578920
  • 胡經(jīng)理
    手機號：13345964880
    QQ：2438244627
  • 周經(jīng)理
    手機號：17757487661
    QQ：1296385441
  • 于經(jīng)理
    手機號：18067160830
    QQ：2088210172
  • 沈經(jīng)理
    手機號：19548299266
    QQ：2662369050
  • 李經(jīng)理
    手機號：13626845108
    QQ：972239479