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31E9
31E9
規格:
價格:
編號:B163701
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
31E9
商品貨號
B163701
Organism
human (B cell); human (leukemia)
Cell Type
hybridoma: T lymphocyte
培養基
Product Format
frozen
Morphology
lymphoblast
Culture Properties
suspension
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease
Allergen
Applications
The cells produce antigen specific GIF.
Disclosure
This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC.
Derivation
Peripheral blood T cells from a patient allergic to Japanese cedar pollen were fused with BUC cells (HPRT - derivative of CEM cells).
Clinical Data
Peripheral blood T cells from a patient allergic to Japanese cedar pollen were fused with BUC cells (HPRT - derivative of CEM cells).
Genes Expressed
Japanese cedar pollen cryj-1 specific human glycosylation inhibiting factor (GIF)
Cellular Products
Japanese cedar pollen cryj-1 specific human glycosylation inhibiting factor (GIF)
Comments
Peripheral blood T cells from a patient allergic to Japanese cedar pollen were fused with BUC cells (HPRT - derivative of CEM cells).
The cells produce antigen specific GIF.
Cryj-1 is a 40000 dalton glycoprotein that constitutes the major allergen in Japanese cedar pollen.
Complete Growth Medium
The base medium for this cell line is ATCC Hybri-Care Medium, Catalog No. 46-X. Hybri-Care Medium is supplied as a powder and should be reconstituted in 1 L cell culture grade water. To make the complete growth medium, add the following components to the base medium:
  • fetal bovine serum to a final concentration of 10%
  • 1.5 g/L sodium bicarbonate for use with 5% CO2 in air atmosphere.
    • Subculturing
    Medium Renewal: Every 2 to 3 days
    Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
    Name of Depositor
    La Jolla Institute for Allergy and Immunology
    U.S. Patent Number
    References

    Ishizaka K. Suppressor T-cell hybridoma and production of allergen specific glycosylation inhibiting factor. US Patent 5,565,338 dated Oct 15 1996

    Ishizaka K. Antigen-specific human glycosylation inhibiting factor. US Patent 5,723,582 dated Mar 3 1998

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