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32B11
32B11
規格:
價格:
編號:B163703
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
32B11
商品貨號
B163703
Organism
Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Cell Type
hybridoma: B lymphocyte
培養基
Product Format
frozen
Morphology
lymphoblast
Culture Properties
suspension
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Applications
The antibody reacts with glomalin on the surface of hyphae and spores and also on glomalin sloughed from hyphae.
Glomalin is a unique fungal protein secreted by the root-dwelling Glomales fungi through hairlike filaments called hyphae.
The antibody is used for studies on soil structure; it is useful in ELISA assays to detect glomalin on the surface of hyphae and glomalin in root cortical cells.
Storage Conditions
liquid nitrogen vapor phase
Derivation
Spleen cells were fused with P3-NSI/1-Ag4-1 myeloma cells.
Glomalin is a unique fungal protein secreted by the root-dwelling Glomales fungi through hairlike filaments called hyphae. It is found worldwide and it may the primary glue that holds soil together.
Genes Expressed
immunoglobulin; monoclonal antibody; against glomalin (a glycoprotein in soil)
Cellular Products
immunoglobulin; monoclonal antibody; against glomalin (a glycoprotein in soil)
Comments
Animals were immunized with freshly collected Glomus intraradices FL208 spores.
Spleen cells were fused with P3-NSI/1-Ag4-1 myeloma cells.
The antibody reacts with glomalin on the surface of hyphae and spores and also on glomalin sloughed from hyphae.
The antibody is slightly cross-reactive with Leptospharia korrea.
Glomalin is a unique fungal protein secreted by the root-dwelling Glomales fungi through hairlike filaments called hyphae. It is found worldwide and it may the primary glue that holds soil together.
The antibody is used for studies on soil structure; it is useful in ELISA assays to detect glomalin on the surface of hyphae and glomalin in root cortical cells.
Complete Growth Medium
RPMI 1640 medium with 2 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate, 4.5 g/L glucose, 10 mM HEPES, and 1.0 mM sodium pyruvate and supplemented with 0.1 mM hypoxanthine and 0.016 mM thymidine, 90%; fetal bovine serum, 10%
Subculturing
Protocol: Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 1 to 2 X 10(5) viable cells/ml. Attached cells may be subcultured by scraping. Maintain cell density between 1 X 10(5) and 1 X 10(6) viable cells/ml.
Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density)
Cryopreservation
Freeze medium: culture medium 92.5%; DMSO, 7.5%
Storage temperature: liquid nitrogen vapor phase
Culture Conditions
Temperature: 37.0°C
Isotype
IgM; kappa light chain
Name of Depositor
SF Wright
Deposited As
mouse (B cell); mouse (myeloma)
References

Wright SF, et al. Time-course study and partial characterization of a protein on hyphae of arbuscular mycorrhizal fungi during active colonization of roots. Plant Soil 181: 193-203, 1996.

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