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331.12
331.12
規格:
價格:
編號:B163705
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
331.12
商品貨號
B163705
Organism
Rattus norvegicus (B cell); Mus musculus (myeloma), rat (B cell); mouse (myeloma)
Cell Type
hybridoma: B lymphocyte
培養基
Product Format
frozen
Morphology
lymphoblast
Culture Properties
suspension
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease
Lymphoma
Applications
At the ATCC tested and found negative for ectromelia virus (mousepox).
Clinical Data
The line was cured of a mycoplasma infection by treatment with BM-cycline and was recloned by the donor.
Genes Expressed
immunoglobulin; monoclonal antibody; against mouse IgM (mu heavy chain)
Cellular Products
immunoglobulin; monoclonal antibody; against mouse IgM (mu heavy chain)
Comments
Animals were immunized with W279 B lymphoma cells.
The line was cured of a mycoplasma infection by treatment with BM-cycline and was recloned by the donor.
At the ATCC tested and found negative for ectromelia virus (mousepox).
Complete Growth Medium
The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density)
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 x 10 exp5 cells/ml and maintain between 1 x 10 exp5 and 1 x 10 exp6 cells/ml.
Cryopreservation
culture medium 95%; DMSO, 5%
Culture Conditions
Temperature: 37.0°C
Isotype
IgG2b
Name of Depositor
PW Kincade
Deposited As
rat (B cell); mouse (myeloma)
References

Kincade PW, et al. Monoclonal rat antibodies to murine IgM determinants. J. Immunol. Methods 42: 17-26, 1981. PubMed: 6165776

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