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356-1
356-1
規(guī)格:
價格:
編號:B163716
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產品名稱
356-1
商品貨號
B163716
Organism
Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Cell Type
hybridoma: B lymphocyte
培養(yǎng)基
Product Format
frozen
Morphology
lymphoblast
Culture Properties
suspension
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Applications
The antibody reacts with a moderately conserved epitope on the JVB strain of CB4.
The antibody also reacts with myocardium from several species including mouse, guinea pig, chicken and rabbit.
Neutralizes many human isolates of CB4.
Tested and found negative for ectromelia virus (mousepox).
Derivation
Spleen cells were fused with P3X63Ag8.653 myeloma cells.
Genes Expressed
immunoglobulin; monoclonal antibody; against coxsackievirus B4 (CB4); also reacts with heart muscle (myocardium); neutralizing
Cellular Products
immunoglobulin; monoclonal antibody; against coxsackievirus B4 (CB4); also reacts with heart muscle (myocardium); neutralizing
Tumorigenic
Yes
Effects
Yes, in BALB/c mice
Comments
Animals were immunized with coxsackievirus B4 (JVB strain, ATCC VR 144).
Spleen cells were fused with P3X63Ag8.653 myeloma cells.
The antibody reacts with a moderately conserved epitope on the JVB strain of CB4.
The antibody also reacts with myocardium from several species including mouse, guinea pig, chicken and rabbit.
Neutralizes many human isolates of CB4.
Tested and found negative for ectromelia virus (mousepox).
Complete Growth Medium
Culture Medium: Dulbecco's modified Eagle's medium (containing 1 mM sodium
pyruvate) with 4.5 g/L glucose, 90%; fetal bovine serum, 10%.
Subculturing
Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
Isotype
IgG2a; kappa light chain
Name of Depositor
BS Prabhakar
Deposited As
mouse (B cell); mouse (myeloma)
References

Prabhaker BS, et al. High frequency of antigenic variants among naturally occurring human Coxsackie B4 virus isolates identified by monoclonal antibodies. Nature 300: 374-376, 1982. PubMed: 6183593

Prabhaker BS, et al. Detection of conserved and nonconserved epitopes on Coxsackievirus B4: frequency of antigenic change. Virology 146: 302-306, 1985. PubMed: 2413617

Ungar BL, et al. Enzyme immunoassay detection of immunoglobulin M and G antibodies to Cryptosporidium in immunocompetent and immunocompromised persons. J. Infect. Dis. 153: 570-578, 1986. PubMed: 3950440

Webb SR, et al. Neutralization epitope diversity of Coxsackievirus B4 isolates detected by monoclonal antibodies. J. Med. Virol. 20: 9-15, 1986. PubMed: 2428930

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